Development of novel chemical mutagenesis assay using budding yeast

排除导致DNA突变的化学物质对于防止细胞肿瘤再生很重要。使用转基因细菌的AMS测试已被广泛用于监测化学品的DNA诱变。然而，这种检测方法可能不适用于检测只对真核生物产生DNA诱变剂的化学物质。我们利用萌芽酵母作为真核生物(AMS酵母)，建立了新型的AMS检测方法。AMS酵母具有编码蛋白质泵的基因缺失，以排除细胞中的化学物质，并可以监测化学物质引起的DNA突变。利用该AMS发芽酵母菌，不仅可以检测到传统AMS试验所检测到的DNA诱变剂，还可以检测到致DNA诱变剂仅限于真核生物的化学诱变剂。

Exclusion of chemicals to cause DNA mutagenesis is important to prevent the incidence of cellular tumoregenesis. The AMS test using genetically-modificated bacteria has been widely employed to monitor DNA mutagenesis of chemicals. However, this assay may not work to detect the chemicals to cause DNA mutagens for eukaryote only. We developed the novel AMS test using the budding yeast as a eukaryote (the AMS yeast). The AMS yeast has the deletion of genes encoding protein pump to exclude chemicals from cells and can monitor the DNA mutagenesis caused by chemicals. Using this AMS budding yeast strain, we can detect DNA mutagens has been detected by traditional AMS test only, but also chemiclas to cause DNA mutagen restricted to eukaryote.