Functional Analysis of Influenza Virus RNA Polymerase usingcompound libraries

从化合物文库中，我们试图分离出抑制流感病毒RNA聚合酶功能的候选化合物，并利用化学生物学方法阐明它们如何抑制聚合酶。通过计算机分析和对感染的抑制作用筛选出含有300万个化合物的文库。最后，我们成功地分离出候选化合物和针对它们的突变病毒。现在，我们正在更详细地分析突变的功能。

From compound libraries, we tried to isolate the candidate compounds which inhibit the function of influenza virus RNA polymerase and to clarify how they inhibit the polymerase using a chemical biology approach. We screened the libraries containing 3 million compounds by in silico analysis and the inhibitory effect on infection. Finally, we succeeded to isolate candidate compounds and the mutant viruses against them. Now, we are analyzing the function of mutations in more detail.