Developing the high sensitive immunochromatograhy with a newl method for improving immunofluorescent signals

在本研究中，我们专注于开发荧光增强方法（IMPACTag）以提高MUSTag灵敏度的具体目标，并将该方法应用于免疫层析和设备。首先，我们尝试开发IMPACTag，但灵敏度不足以达到我们的目标，我们改变了使用荧光珠代替IMPACTag的策略。在我们的研究期间，我们成功地开发了高灵敏度的免疫层析（IC）尖端和设备与公司合作。在检测流感病毒方面，集成电路芯片和装置检测病毒的灵敏度比传统方法高100倍。此外，我们还成功地开发了检测乳头瘤病毒L1蛋白抗体的检测系统。该系统可用于评估接种后的宫颈癌疫苗效果。

In this study, we focused on the specific aim to develop the fluorescent enhancement methods (IMPACTag) for improving the MUSTag sensitivity and also apply this methods to Immunochromatography and the devices.First, we tried to develop the IMPACTag, but the sensitivity was not enough to our goal, and we changed the strategy to use fluorescent beads instead of IMPACTag. During our research term, we succeeded in developing the high sensitive immunochromatography (IC) tips and the device in collaboration with companies. For detection of influenza viruses, the IC chip and the device could detect viruses 100 fold more sensitive than conventional methods. Furthermore, we also succeeded in the developing the assay system for detecting antibodies against papilloma virus L1 proteins. the system will be available for the evaluation of the cervical cancer vaccine effect after the inoculation.