Organ Engineering by Decellularized Scaffold for Transplant and Regenerative Therapy

干细胞工程的最新技术为体外研究复杂的生理过程以及制造用于移植的整个器官的潜力提供了新的可能性。人类诱导性多能干细胞在体外已经取得了巨大的进展，可以分化出相对功能性的体细胞，我们应该进入下一个阶段，利用可行的动物模型来研究这些细胞的临床应用。因此，我们开发了一种新的技术，使用脱细胞三维支架来产生可移植的全肝，该全肝可以用合适的细胞类型进行再细胞化。它可以保留所有必要的线索，组织再生与有效的血液供应，通过血管通路在体内。对于临床应用，应应用于临床前实验性大动物模型。我们相信这项技术可能有助于对器官再生以及药物代谢和毒性开辟新的见解。

Recent technology in stem cell engineering has raised new possibilities for the study of complex physiological processes in vitro, as well as the potential to fabricate entire organs for transplantation. After the huge progress in vitro using human-induced pluripotent stem cells which can differentiate relatively functional somatic cells, we should go for the next stage to investigate clinical applications of those cells using feasible animal models. Therefore, we have been developed a new technology using acellular three-dimensional scaffolds to generate transplantable whole liver which can be recellularized with appropriate cell types. It can retain all the necessary cues of tissue regeneration with efficient blood supply by suturing vascular access in vivo. For the clinical application, it should be applied to preclinical experimental large animal models. We believe that this technology may help to open new insight into organ regeneration as well as drug metabolism and toxicity.