Functional analysis on the molecular and cellular biology of IPAS which induces neuronal cell death and production of its knockout mouse

通过对IPAS诱导细胞死亡的调控机制分析，我们发现IPAS与促生存Bcl-2家族蛋白如Bcl-xL结合。此外，IPAS以线粒体解偶联剂、cccp依赖的方式与PINK1和Parkin结合，导致IPAS的泛素化和降解。为了阐明IPAS在体内神经元细胞死亡中的生理功能，我们进行了IPAS基因敲除小鼠的制备。我们已经获得了IPAS嵌合体小鼠。

As a result of the analysis about regulatory mechanism of IPAS- induced cell death, we have shown that IPAS was bound to pro-survival Bcl-2 family proteins, such as Bcl-xL. Furthermore, IPAS was bound to PINK1 and Parkin in a mitochondria uncoupler, CCCP-dependent manner, leading to ubiquitination and degradation of IPAS. To elucidate the physiological function of IPAS in neuronal cell death in vivo, we performed the production of IPAS knockout mouse. We have already obtained IPAS chimera mice.