Elucidation of mechanisms of the pathogenesis in Kawasaki disease by auto-reactive antibody

虽然免疫系统功能障碍参与了川崎病的发病，但其病因仍有待明确。我们研究了KD患者的自身抗体，这些抗体经常在自身免疫性疾病、血管炎和动脉炎患者的血清中发现。我们对自身抗体抗原进行了蛋白质组学分析，并确定了几个候选抗原。通过酶联免疫吸附试验，我们发现KD患者血清中auto的滴度明显高于年龄匹配的对照组。大鼠心脏切片免疫组化结果显示，在心肌细胞内，抗原与肌球蛋白轻链共定位。患者血清免疫染色也给出了类似的结果。这些结果提示自身抗体可能是KD的诊断标志物。

Although the involvement of immune system malfunction in the onset of Kawasaki disease is suggested, its etiology still remains to be clarified. We investigated autoantibodies in KD patients, which are frequently found in sera from patients with autoimmune diseases, vasculitis and arteritis. We performed proteomic analysis for antigens of autoantibodies, and identified several candidate antigens. By enzyme-linked immunosorbent assay, we found that the titer of an auto in sera from KD patients was significantly higher than those from age-matched controls. Immunohistochemitry of rat heart thin sections revealed that an antigen was colocalized with myosin light chain in cardiac myocytes. Immunostaining with patient's sera also gave similar results. These results suggest autoantibodies as a possible diagnostic marker for KD.