Application of artificial antibody for an inhibitor against signal transduction

本研究利用酵母主成分分析法，建立了用于人工抗体筛选系统的蛋白质文库。由此，建立了细胞质靶亲和分子的筛选方法。选择细胞内信号转导级联的重要分子Ras和Raf作为靶分子。然后，选择与这些分子有亲和关系的分子，并在细胞质中进行评价。类风湿关节炎作为一种典型疾病，参与了Ras/Raf信号转导的级联反应。在这些条件下，选定的附着体克隆使用滑膜和成骨细胞系进行增殖或细胞因子生产评估。

In this study, protein library using yeast PCA method has been developed for screening system of artificial antibody. Thus, the screening method of cytosolic target-affinity molecules was established. Important molecules of intracellular signal transduction cascade, Ras and Raf, were chosen as target molecules. Then, affinity molecules against these molecules were selected and evaluated in cytosol. As a model disease, rheumatoid arthritis which is involved in Ras/Raf signal transduction cascade. Under these conditions, selected affibody clones were evaluated on proliferation or cytokine-production using synovial and osteoblast cell lines.