Fabrication of biosensor for specific pollutant detection based on the biological magnetic particle synthesis

本研究以趋磁细菌为研究对象，利用趋磁细菌产生磁性颗粒的活性，开发一种用于特定污染物检测的磁性生物传感器。在这里，我们评估了最低抑菌浓度（MIC）和磁铁矿生产力的分布的趋磁细菌生长在各种过渡金属离子和类金属含氧阴离子的存在下。结果表明，细菌磁铁矿中Co、Mn和Cu的掺杂量最高，MIC曲线也依赖于金属离子。此外，对不同条件下生长的细菌进行了磁性测量，揭示了细菌磁性的差异。在此基础上，研制了微生物传感器系统样机。利用本研究的结果和信息，在不久的将来，新型的高特异性检测污染物的生物传感系统将被开发出来。

In this research project, to develop a magnetic biosensor for specific pollutant detection, magnetotactic bacteria was investigated, because of their activity of magnetic particle production. Here we evaluated minimum inhibitory concentration (MIC) and magnetite productivity profiles of the magnetotactic bacteria grown in the presence of various transition metal ions and metalloid oxyanion. As a result, the highest doping of Co, Mn and Cu in bacterial magnetite were successfully found and the MIC profiles were also metal ion dependents. In addition, magnetic measurements of these bacteria grown in different condition revealed the difference of the magnetic property. Based on these information, a prototype of microbial biosensor system has been fabricated. Using the results and information in this study, novel biosensing system for highly specific detection of pollutant will be developed in near future.