Generation of transformants showing mineral-stress tolerance based on the modification of light-harvesting function

光合电子传递链中有许多含铁蛋白参与，缺铁导致光系统II（PSII）和PSI之间电子传递的失衡。我们最近发现，铁缺乏特异性地诱导LHCII家族的基因，该基因编码大麦（大麦）中Lhcb 1的同源物，大麦是一种耐受铁缺乏的植物。在这项研究中，我们证明了一个大比例的HvLhcb 1是稳定的磷酸化，并位于邻近PSI，而不是PSII铁缺乏的条件下。这些结果表明，缺铁大麦中HvLhcb 1的磷酸化诱导了触角与PSII核心的分离，并将蛋白质迁移到PSI，从而优化了电子传递速率。最后，我们通过基因工程改造水稻植物来表达HvLhcb 1基因，并评估该基因是否赋予植物耐受性和/或在各种环境下提高植物生产力。

There are many iron-containing proteins working in photosynthetic electron transport chain so that iron deficiency causes the imbalance in the electron transport between photosystem II (PSII) and PSI. We have recently found that iron-deficiency specifically induces a gene of the LHCII family that encodes a homolog of Lhcb1 in barley (Hordeum vulgare), a plant that is tolerant to Fe deficiency. In this study, we demonstrate that a large proportion of HvLhcb1 was stably phosphorylated and located adjacent to PSI rather than PSII under Fe-deficient conditions. These results suggested that the phosphorylation of HvLhcb1 in Fe-deficient barley induces the disconnection of the antenna from the PSII core and migration the protein to PSI, resulting the optimization of the electron transport rate. Finally, we genetically engineered rice plants to express the HvLhcb1 gene and evaluated whether the gene confers tolerance to plants and/or improves plant productivity under various environments.