Regulatory mechanism of strigolactone production under nitrogen deficiency

LC-MS/MS分析表明，水稻体内的SL水平随着氮素缺乏的响应而升高。QRT-PCR分析表明，在缺氮条件下，SL生物合成基因(D10、D17、D27和OsMAX1s)高表达，而SL信号转导基因(D3和D14)不表达。我们认为，水稻并没有改变对SL感知的敏感性，而是增加了SL的产量，以响应氮素缺乏。此外，我们利用基因芯片寻找新的SL生物合成和代谢基因，并筛选出与SL生物合成基因表达模式相似的基因。

SL analysis by LC-MS/MS showed that SL levels elevated in response to nitrogen deficiency in rice. SL-related gene expression analysis by qRT-PCR demonstrated that SL biosynthetic genes (D10, D17, D27, and OsMAX1s) highly expressed under nitrogen deficiency, but not SL signal transduction genes (D3 and D14). We propose that rice does not change the sensitivity of SL perception but increases SL production in response to nitrogen deficiency. In addition, we performed microarray to find new SL biosynthetic and metabolism genes, and screened genes that showed the expression pattern similar to SL-biosynthetic genes.