Spatiotemporal analysis of lipids in avian yolk

在之前的研究中，我们用MALDI成像质谱仪(IMS)可视化了磷脂酰胆碱(PC)的分子种类。它们在鹅黄蛋黄中形成了独特的环状结构，根据PC分子物种中包含的脂肪酸类型进行了分类。PC分子物种形成的环结构与已知的由暗带和光带形成的环结构不同，在本研究中，我们试图用MALDI-IMS来可视化其他磷脂。显示了磷脂酰乙醇胺(PE)、磷脂酰肌醇(PI)、溶血磷脂酰胆碱(LPC)、溶血磷脂酰乙醇胺(LPE)和鞘磷脂(SM)的分子种类。Pe和PI分子物种也在蛋黄中形成了特有的环状结构。此外，SM分子种类主要分布在生发泡和后胚层。

In the previous study, we visualized phosphatidylcholine (PC) molecular species by MALDI imaging mass spectrometry (IMS). They formed characteristic ring structures in the quail yellow yolk that were categorized by the type of fatty acid contained in the PC molecular species. The ring structures formed by the PC molecular species were distinct from the known ring structure formed by dark and light bands.In this study, we attempted to visualize other phospholipids by MALDI-IMS. Phosphatidylethanolamine (PE), phosphatidylinositol (PI), lysophosphatidylcholine (LPC), lysophosphatidylethanolamine (LPE), and sphingomyelin (SM) molecular species are visualized. PE and PI molecular species also formed characteristic ring structures in the quail yellow yolk. Furthermore, most of SM molecular species are distributed in the germinal vesicle and latebra.