Role of p62 and Nbr1 in regulation of food intake regulation

虽然已知Nbr1具有与p62相似的结构域结构，但其生理功能已被揭示。在本研究中，我们旨在研究Nbr1的功能。我们用WT和p62-KO小鼠建立了WT、p62-KO、WT:Nbr2-KD、p62-KO:Nbr1KD mef（小鼠胚胎成纤维细胞）。我们发现WT:Nbr1-KD细胞的细胞生长速度明显慢于其他细胞。由于总p38和磷酸化p38水平降低，我们推测Nbr1可能调节p38蛋白水平，缺乏Nbr1会导致细胞生长率降低。

Though Nbr1 is known to have similar domain structure as p62, the physiological function has been uncovered. In this study, we aimed to examine the Nbr1 function. We used WT and p62-KO mice to establish WT, p62-KO, WT:Nbr2-KD, p62-KO:Nbr1KD MEFs (mouse embryonic fibroblast). We found the cell growth ratio in WT:Nbr1-KD cells are significantly slower than the other cells. Because total- and phosphorylated-p38 levels were decreased, we speculated Nbr1 has a role to regulate p38 protein level, and Nbr1 deficiency results in the decrease in cell growth ratio.