The role of cyclophilin A in diabetic vascular calcification

动脉钙化可伴随多种心血管疾病，如动脉粥样硬化、糖尿病和肾功能衰竭。血管平滑肌细胞的成骨分化在血管钙化过程中起着重要作用。观察到无机磷酸盐（Pi）增加了rho激酶的激活，这与培养的VSMCs中亲环蛋白A的自分泌、碱性磷酸酶（ALP）活性和钙的积累有关。rho激酶抑制剂预处理可抑制pi诱导的ALP活性和钙积累。这些结果提示，rho激酶的活化和亲环蛋白A的自分泌可能与pi诱导的血管钙化有关。

Arterial calcification is accompanied by several cardiovascular diseases such as atherosclerosis, diabetes mellitus, and renal failure. Osteogenic differentiation of vascular smooth muscle cell (VSMC) plays important role in vascular calcification. It was observed that the inorganic phosphate (Pi) increased rho-kinase activation which is reported to be involved in the autocrine of cyclophilin A, alkaline phosphatase (ALP) activity, and calcium accumulation in cultured VSMCs. The pretreatment with rho-kinase inhibitor inhibited Pi-induced ALP activity and calcium accumulation. From these results, it was suggested that rho-kinase activation and the autocrine of cyclophilin A might be related with Pi-induced vascular calcification.