Identification of the molecule which controls lymphangiogenesis and research of the medical treatment method for lymph node metastasis and lymphedema

为了评估淋巴管生成、淋巴结转移、肺转移，我们建立了荧光素酶稳定表达的人肺癌细胞系（A549）。我们检测了肺癌细胞的胞内信号。我们发现了影响HIF1a活性的化合物，HIF1a对血管生成和淋巴管生成很重要。我们研究了一些化合物对淋巴管生成的抑制和促进作用。将A549荧光素酶稳定表达细胞输注小鼠体内，观察其对肿瘤生长、淋巴管生成、淋巴结转移、肺转移的影响。

For evaluation of lymphangiogenesis, lymph node metastases, pulmonary metastasis, we established the luciferase stability expression human lung cancer cell line (A549). We examined the intracellular signals in lung cancer cells. We identified the compound which had an influence on the activity of HIF1a which was important to angiogenesis and lymphangiogenesis. We examined the effects of some compounds on lymphangiogenesis inhibition and promotion. Also, A549 luciferase stability expression cells were transfused into mice and we examined the effects of these compounds on tumor growth, lymphangiogenesis, lymph node metastases, pulmonary metastasis.