Analysis of immune regulation by dioxin receptor via induction of microRNA

我们证明miR-132/miR-212簇双敲除小鼠比WT小鼠对LPS过敏。我们发现，在LPS刺激下，miR-132/miR-212簇在腹腔巨噬细胞中上调，而在M1或M2巨噬细胞中不上调。与WT细胞相比，lps诱导的miR-132/miR-212簇上调在Ahr敲除的腹膜巨噬细胞中被抑制。我们还证明IL-6可能是miR-132/miR-212簇的直接靶点。因此，ahr依赖性miR-132/miR-212负调控lps诱导的腹腔巨噬细胞中的IL-6。

We demonstrated that miR-132/miR-212 cluster double knock-out mice are hypersensitive to LPS than WT mice. We showed miR-132/miR-212 cluster is up-regulated in peritoneal macrophages but not in M1 or M2 macrophages in the stimulation of LPS. LPS-induced upregulation of miR-132/miR-212 cluster is suppressed in Ahr knock-out peritoneal macrophages compared to WT cells. We also demonstrated that IL-6 could be a direct target of miR-132/miR-212 cluster. Therefore, Ahr-dependent miR-132/miR-212 negatively regulates LPS-induced IL-6 in peritoneal macrophages.