Identification of biomarkers by RNAi screening that predict efficacy of chemotherapy in colon cancer

在这项研究中，我们利用全基因组RNA干扰筛选，探索下调促进结肠癌细胞对抗癌药物产生耐药性的基因。我们从DLD-1中获得了12个对伊立替康耐药的亚型。我们确定了三个基因，它们的表达实际上在耐药亚系中受到抑制。所鉴定基因的抑制导致DLD-1细胞对伊立替康产生耐药性。在这三个基因中，我们重点研究了编码组蛋白甲基转移酶的基因。当该基因被敲除时，细胞生长受到抑制，伊立替康诱导的细胞凋亡受到抑制。

In this study, using genome-wide RNA interference screening, we explored genes whose downregulation promote colon cancer cells resistant to anticancer drugs.We obtained a dozen sublines from DLD-1 that were resistant to irinotecan. We identified three genes whose expression were actually suppressed in the resistant sublines. The suppression of identified genes caused DLD-1 cells resistant to irinotecan. Among those three genes, we focused on the gene encoding histone methyltransferase. When this gene was knocked down, cell growth was inhibited and apoptosis induced by irinotecan was suppressed.