共生性渦鞭毛藻類　Ａｍｐｈｉｄｉｎｉｕｍｓｐ．　のゲノムおよび毒素生合成

我们完成了吉氏双歧杆菌的基因组测序，并利用整合组学的方法，研究了次生代谢物生物合成的机制。我们利用来自Illumina和PacBio的测序数据，将基因组学、转录组学和微RNA组学相结合。我们确定了产生次生代谢物所需的关键酶。我们还发现，在营养条件下(硝酸盐)，次生代谢物的生物合成由于miRNAs的可能作用而下调。这些信息有助于了解有害藻华的形成。我们还确定了几种影响其表达的分子事件，如次生代谢物基因的选择性剪接。

We completed the genome of Amphidinium gibbosum and using an integrated omics approach, we surveyed the mechanisms of secondary metabolite biosynthesis. We combined genomics, transcriptomics and microRNAomics using sequencing data from Illumina and Pacbio.We identified key enzymes needed to make secondary metabolites.We also found that under nutrient conditions ( nitrate), secondary metabolites biosynthesis is downregulated because of the probably role of a miRNA.Such information is helpful in helping understand harmful algal bloom formation.We also identified several molecular events such as alternative splicing in secondary metabolite genes, that affect their expression.Taken together, we propose 2 strategies for the synthesis of secondary metabolites in dinoflagellates that include iterative use of enzymes.