Cellular resolution macro analysis tools for functional and structural brain imaging

于2021财政年度，我们成功开发一款原型荧光显微镜，用于全脑或感兴趣的组织块的自动连续切片和高分辨率宽视野块面成像。于2022财政年度，对正常小鼠大脑进行了定量分析的应用。研究如预期顺利进行。

The purpose of the proposed research was to develop open source microscopy tools for 3D brain imaging using novel light source for fluorescence microscopy in the deep ultraviolet spectrum.In FY2021, a prototypical fluorescence microscope for automated serial slicing and high resolution widefield block-face imaging across the whole brain or tissue block of interest was successfully developed. A manuscript submitted on the technical aspects is currently in second phase of revision.In FY2022, the applications for quantitative analysis was carried out on normal mouse brains. The study is running smoothly as expected.