High-resolution spatiotemporal analysis of harmaline-induced tremor and inferior olive activity in living mice

在过去的一年里，我们专注的研究团队在阐明震颤相关疾病的运动学和神经运动机制方面取得了显著进展。我们已经成功地启动了一个项目，以确定GCamP6在下橄榄(IO)中病毒表达的安全条件，这在理解骆驼蓬碱诱发的小鼠震颤中发挥了关键作用。这项工作的发现将在2023年8月在仙台举行的备受尊敬的JNS会议上公布。同时，我们扩展了我们的实验装置，以评估骆驼蓬碱诱发的震颤小鼠执行各种运动任务，如高速运动、平衡和3D空间探索。此外，我们还将这些复杂的自然主义行为任务与小脑核活动的同步记录结合在一起，于2023年4月完成了第一批实验。我们的进展表明，我们坚定不移地致力于克服研究震颤相关疾病的挑战。

In the past year, our dedicated research team has made noteworthy progress in elucidating the kinematic and neuromotor mechanisms of tremor-related disorders. We have successfully initiated a project to determine safe conditions for viral expression of GCamP6 in the inferior olive (IO), which plays a critical role in understanding harmaline-induced tremor in mice. This work's findings will be presented at the esteemed JNS conference in Sendai, August 2023. Concurrently, we expanded our experimental setup to assess harmaline-induced tremor in mice performing various motor tasks, such as high-speed locomotion, balancing, and 3D space exploration. Furthermore, we have integrated these intricate naturalistic behavior tasks with simultaneous recording of cerebellar nuclear activity, completing the initial full batch of experiments in April 2023. Our progress demonstrates our unwavering commitment to overcoming challenges in studying tremor-related disorders.