Detection and quantification of endocannabinoids by LC-MS/MS

作为第一个资助期的延续，我们将为项目研究小组测量主要的内源性大麻素(ECB)。此外，我们建议将我们的研究扩展到“超越CBS”，包括更多的ECB相关分析物，并开发新类型生物样本的提取方法。我们进一步打算“超越ECS”，并使用脂组学技术更全面地研究ECS与其他脂质信号系统和一般细胞脂质代谢的相互作用。AB SCIEX 5500 QTRAP是一种三-四极线性离子陷阱混合仪器，其快速扫描速度和高灵敏度已被证明对欧洲央行的MRM(多反应监测)定量具有非常重要的价值。关于“Beyond ECS”分析，我们将把仪器独特的线性离子捕获能力与依赖信息的获取结合起来，以建立不同类型的脂组工作流程(例如，有针对性的方法与非有针对性的方法)。

In continuation of the first funding period, we will measure the major endocannabinoids (eCBs) for the project research groups. In addition, we propose to extend our investigations “beyond CBs" by including additional eCB-related analytes and to develop extraction methodologies for new types of biological samples. We further intend to go "beyond the eCB system (ECS)” and to use lipidomic technologies for more comprehensive investigations of the interactions of the ECS with other lipid signaling systems and the general cellular lipid metabolism. The fast scan speed and high sensitivity of the AB SCIEX 5500 QTRAP, a triple-quadrupole linear ion trap hybrid instrument, have already been proven to be invaluable for eCB quantification by MRM (multiple-reaction-monitoring). Regarding "beyond ECS" analyses, we will combine the instrument's unique linear ion trap capabilities with information-dependent acquisition to establish different types of lipidomic workflows (e.g., targeted versus non-targeted approaches).