The foundness of colours and complex species taxonomy- a DNA based approach for revealing the genealogical structure in the European rose chafer (Cetonia aurata L.)

使用分子来解决分类学和种群结构问题被认为是研究生物系统是如何组织和推断其历史的最有前途的方法。除了传统的基于形态的分类学和类型学分类，我们打算克服这种方法的一些局限性，使用线粒体和核序列来重建欧洲玫瑰金龟子的系统地理，这是一个自然保护问题的闪耀物种，也是中欧最具吸引力的金龟子之一。先前的初步序列分析揭示了线粒体DNA中一些独特的遗传结构，这既不符合迄今定义的亚种，也不符合不同的地理分布。通过对该物种整个分布范围内的不同种群相对于众多现有的颜色形式和密切相关物种的全面采样，我们不仅将揭示该物种的历史生物地理，包括它们在冰川时期的避难地，而且还将试图探索目前的颜色多态是否在某种程度上与该物种的遗传结构有关。此外，我们将探索到目前为止使用的形态特征如何与获得的树木模式相关。基于从更广泛的系统发育分析中对树的校准，我们计划在我们的重建中引入一个时间尺度。这些结果有望为我们提供有关该物种复合体的多样化模式以及导致该物种遗传结构的潜在过程的有用信息。此外，我们希望在物种分类学(物种划界)的关键和极端案例的背景下，例如在密切相关的类群、异源和毗邻种群和亚种中，更详细地体验DNA分类学的方法。

Using molecules to address questions of taxonomy and population structure is perceived as the most promising approach to investigate how biological systems are organized and to infer about their history. Alternativly to traditional morphology-based taxonomy and typological classification, we intend to overcome some limitations of this approach using mitochiondrial and nuclear sequences to reconstruct the phylogeography of the European rose chafer Cetonia aurata, a flaship-species for issues of natural conservation and one of the most attractive scarab beetles in central Europe. Previous prelimiary sequence analyses have revealed some distinct genetic structure in mitochondrial DNA which is neither consistent with subspecies so far defined nor with different geographical occurrences. With a comprehensive sampling of the different populations of the entire distribution range of the species with respect to the numerous existing colour forms, and closely related species and we will uncover not only the historical biogeography of the species including their refugial areas during glaciation periods but also try to explore whether the present colour polymorphism is coupled in some exctend to the genetic structure in the species. In addition, we will explore how so far used morphological charaters correlate with the tree patterns obtained. Based on calibration of the tree from a wider phylogenetic analaysis we plan to introduce a time scale to our reconstructions. The results will provide us hopefully useful information about patterns of diversification in this species complex and the underlying processes leading to the genetic structure of the species. Furthermore, we hope to experience methodologies of DNA taxonomy more in detail in a context of critical and extreme cases of species taxonomy (species delimitation) such as in closely related taxa, alloand parapatric populations and subspecies.