Oral nanoparticle formulations for allergy treatment via efficient induction of immune tolerance

今年的研究目的是改进耐受性纳米颗粒(TNP)，以诱导免疫耐受和防止过敏反应1。通过对TNP的制备、粒度控制和溶出度实验的研究，确定了本年度TNP的基本性质。关于TNP与细胞的相互作用，通过与BMDCs的共培养观察，证实TNP可以被BMDCs吞噬，并能改变BMDCs的分化方向。此外，我们还证实了TNP具有一定的T细胞刺激能力，这对于建立免疫耐受是至关重要的。在动物实验方面，我们进行了安全性评价，确认了TNP的安全性，没有引起过敏反应。随后，我们将TNP应用于OVA过敏模型小鼠的预防实验。实验结果表明，TNP能有效预防过敏反应。这意味着我们开发的TNP可以成为一种新型的过敏治疗方法。本研究的意义在于通过TNP制剂诱导免疫耐受，在不影响正常免疫反应的情况下防止过敏反应。与传统的变态反应治疗方法相比，TNP具有制备容易、见效快等优点，可以极大地提高患者治疗的便利性和生活质量。总之，今年的研究成果为免疫耐受研究和变态反应治疗提供了新的思路和方法，在临床治疗中具有潜在的应用价值。

The aim of this year's research was to improve the tolerogenic nanoparticle(tNP) for inducing immune tolerance and preventing allergic reactions.1. Through research on the preparation of the tNP, size control, and dissolution experiments, we successfully determined the basic properties of the tNP in this year.2. Regarding the interaction between tNP and cells, through co-culture observations with BMDCs, we confirmed that the tNP can be phagocytosed by BMDCs and can change the differentiation direction of BMDCs. Additionally, we confirmed that the tNP has certain T cell stimulation capability, which are crucial for establishing immune tolerance.3. In terms of animal experiments, we conducted safety evaluation and confirmed the safety of the tNP without causing anaphylaxis. Subsequently, we applied the tNP in prevention experiments on OVA allergy model mice. The experimental results showed that the tNP can effectively prevent allergic reactions. This means that the tNP we developed can become a new type of allergy treatment.The significance of this research lies in inducing immune tolerance through tNP preparation to prevent allergic reactions without affecting normal immune responses. Compared to traditional allergy treatment methods, tNP has the advantages of being easy to prepare and quick to take effect, which can greatly improve the convenience of patient treatment and quality of life. Overall, the research results of this year provide a new idea and method for immune tolerance research and allergy treatment, with potential application value in clinical treatment.