Functions of the major membrane microdomain protein reggie/flotillin in formation of highly mobile forms of Wnt and Hedgehog morphogens in Drosophila

脂质修饰的形态因子Wnt和Hedgehog通过至少两种途径在组织中分泌和传播。第一个结果是形成一个流动性差的形态原库，并激活短程靶基因。第二种形成了激活远程目标所需的更具流动性的形态池。我们已经证明，果蝇的第二种分泌途径依赖于一种主要的膜微结构域组织蛋白reggie-1/ flotilin -2的功能。当前项目的目标是：a)了解Wnt和Hh形态因子的依赖于区域的高度移动形式的分子性质；B)在超微结构水平研究形态素分泌途径及其对reggie-1的依赖性；c)阐明果蝇翅膀发育过程中reggie-1参与的其他信号通路。

The lipid-modified morphogens Wnt and Hedgehog are secreted and spread through the tissue by at least two pathways. The first results in formation of a poorly mobile morphogen pool, and activates short-range target genes. The second forms a more mobile morphogen pool required for activation of longrange targets. We have demonstrated that this second secretion route depends in Drosophila on the function of one of the major membrane microdomain organizing proteins, reggie-1/flotillin-2. The aims of the current project are: a) to understand the molecular nature of the reggie-dependent, highly mobile form of the Wnt and Hh morphogens; b) to investigate the morphogen secretion routes and their dependence on reggie-1 at the ultrastructural level; and c) to elucidate involvement of reggie-1 in other signaling pathways during Drosophila wing development.