Molecular assembly of becteriochlorophills of light-harvesting model plypeptides and its characterization

有趣的是，光合细菌的光收集（LH）多肽根据多肽与BChla之间的合作相互作用来组织bachio叶绿素a （BChla）复合物，从而可能发生有效的卟啉的能量转移和电子转移过程。在本文中，我们现在展示了BChla及其类似物的分子组装，并通过在n-辛基- β - d -葡萄糖吡喃苷（OG）胶束中使用具有光的多肽和合成的疏水α -螺旋多肽来深入了解LH多肽的氨基酸残基对光合细菌LH复合物形成的影响，如下所示。1)研究了氟烷、氯仿和己醇对正辛基- β - d -葡萄糖吡喃苷（OG）中光合细菌重组光收集（LH）多肽-细菌叶绿素a（BChla）复合物的影响，以揭示麻醉剂对膜蛋白复合物的作用。光晕对光合细菌的复合体和染色质的变性作用最强。用热力学方法分析了其对LH-Bchl - a配合物稳定性的影响，并对其变性效果进行了比较。变性效果从大到小依次为氟烷b>氯虫b> b>己醇。2)利用疏水的1 - a-螺旋多肽和2 - a-螺旋带二硫键的多肽，研究了细菌叶绿素（B - Chla）及其类似物的分子组装。分子组装的关键是利用疏水2a-螺旋多肽对形成光合细菌LH1复合体所需的氨基酸残基进行检测。在n-辛基-b- d -葡萄糖吡喃糖苷胶束中，细菌叶绿素/ lh模型多肽复合物的光谱性质与光合细菌的捕光多肽的光谱性质类似，特别是当2 - a-螺旋多肽存在时。少

It is interesting to note that the light-harvesting (LH) polypeptides of photosynthetic bacteria organize bachteriochlorophyll a ( BChla ) complex according to cooperative interactions between the polypeptide and BChla so that an efficient energy-transfer and electron-transfer processes of porphyrins may occur. In this paper, we now demonstrate molecular assemblies of BChla and its analogue and by using light-havesting polypeptide and synshtetic hydrophobic alpha-helix polypeptides in n-octyl-beta-D-glucopyranoside (OG) micelle to provide an insight into the effect of amino acid residues of the LH polypeptide on formation of the LH complex of photosynthetic bacteria as following.1) The effect of halothane, chloroform and hexanol on reconstituted light-harvesting (LH) polypeptides-bacteriochlorophyll a(BChla) complex from photosynthetic bacteria in n- octyl- beta -D-glucopyranoside (OG) was examined to provide insight into the action of anaesthetics to the membrane protein complex. Halo … More thane has the strongest denaturing effect for both the reconstituted complex and the chromatophore from photosynthetic bacteria. Furthermore, the effect was analyzed by thermodynamics on the stability of the LH-Bchl a complex and the comparison of the denaturing effect was made. The denaturing effect became large in the following order, halothane > chlorohorm >> hexanol.2) We demonstrated molecular assemblies of bacteriochloro-phyll (B Chla) and its analogues by using hydrophobic 1 a-helix polypeptides and 2 a-helix polypeptides with disulfide-linkage. The key to the molecular assembly is a ready availability of examination of the amino acid residue necessary for forming LH1 complex of photosynthetic bacteria by using hydrophobic 2a-helix polypeptides. Spectroscopic properties of bachteriochlorophylls / LH-model polypeptides complex in n-octyl-b-D-glucopyranoside micelle were analogous to those obtained using light-harvesting polypeptides from photosynthetic bacteria especially when 2 a-helix polypeptides were present. Less