DEVEL0PMENT OF IN VIVO MODEL OF HUMAN HAIR AND ITS APPLICATION

我们尝试了人毛囊的冷冻保存，以建立人头发的活体实验系统。用眼科剪、钳分离出的人头皮毛囊分为两组(冷冻毛发92根，非冰冻毛发58根)。一组立即移植到BALBIcA-nu、SCID小鼠体内，另一组在含有二甲基亚砜的细胞库中浸泡，放入微管中，在-80ﾟC的冷冻箱中保存1周以上。用21-25Gage注射针在小鼠背部皮肤上穿孔，每根毛干插入皮肤孔内，用生物物理胶粘剂固定。冻存组移植成功率为27.2%，未冻存组为27.9%。两组间未见组织学差异。这些结果表明，用通常的方法冷冻保存人类毛囊相对容易。使用这种方法，有可能计划对人体毛发进行体内研究的系统实验。考虑到移植的成功率，将10根人类毛发移植到一只小鼠体内，可以得到一只有两根人类毛发的小鼠。因此，该方法具有一定的实用价值。利用该系统，我们尝试建立了保存了不同性别、不同年龄、不同地点、不同疾病等多种冷冻保存的人发库，但由于近年来尸检率的下降和知情同意的困难，目前仅保存了11例。五分之二的头发脱落，其他一些头发显示出组织学上的退化。但其中一些没有表现出组织学变化。因此，需要进一步的研究来阐明睾酮的作用。

We tried frozen preservation of human hair follicles to establish the in vivo experimental system of human hairs. The isolated human hair follicles from the scalp by ophthalmologic scissors and forceps were separated to two groups (92 frozen hairs, 58 non-frozen hairs). One group was immediately transplanted to BALBIcA-nu, scid mice and another group was immersed in the Cell Stock Medium with DMSO , put into microtube and kept in the Deep Freezer at -80ﾟC more than 1 week. The mouse skin at the dorsal trunk was perforated by the 21-25 gage injection needle and each hair shaft was inserted into the skin hole and fixed by the biophysical adhesive glue. Average success rate of transplantation was 27.2 % in the frozen group and 27.9% in the non-frozen group. Histological differences were not revealed between two groups. These results showed that human hair follicles are relatively easily frozen-preserved by the usual methods. Using this method, it may be possible to plan systematic experiments of in vivo studies of human hairs. Considering the success rate of transplantation, a mouse with two human hairs are obtained when 10 human hairs are transplanted in a mouse. Therefore, this method is of practtical use. Using this system, we tried to establish "human hair bank" with many frozen preserved human hairs with different sexes, ages, sites, diseases and etc. But only 11 cases are now preserved because of recent decline of autopsy rate and the difficult informed consent.Next, using this experimental system, we tried to analyze the effects of testosterone to transplanted human hairs. Two out of five hairs fell off and some of others showed histological degeneration. But some of them showed no histological changes. Therefore, further studies are needed to elucidate the effects of testosterone.