Clinical roles of plasma phosphatidylcholine hydroperoxide-reducing activity in the development of pre-eclampsia

血浆脂质过氧化物是动脉粥样硬化形成的危险因素，因为脂质过氧化物攻击内皮细胞。我们发现，血浆具有脂质过氧化还原活性，而不是血浆谷胱甘肽过氧化物酶，一种主要的脂质过氧化还原酶。在这项研究中，我们初步纯化了这种脂质过氧化还原蛋白从人血浆中，并成功地分离载脂蛋白A1和B100作为磷脂酰胆碱氢过氧化物还原蛋白。为了阐明该活性的病理生理作用，我们开发了一种使用合成的1-甲酰基-2-壬油酰基-磷脂酰胆碱氢过氧化物（PLPC-OOH）作为底物来测量血浆磷脂酰胆碱氢过氧化物还原活性的测定方法。用本法测定了53例正常妊娠、11例子痫前期患者、18例子痫前期患者和11例子痫前期高危人群血浆中PLPC-OOH还原活性。PLPC-OOH还原活性在高危组高于正常妊娠，而脂质过氧化物水平在先兆子痫患者中更高。目前的结果表明PLPC-OOH还原活性在消除血浆脂质氢过氧化物中的重要作用，并进一步参与先兆子痫的发展。

Plasma lipid peroxide has been known as risk factor for atherogenesis, because lipid peroxide attacks endothelial cells. We found that blood plasma possess lipid peroxide reducing activity other than plasma glutathione peroxidase, a major lipid peroxide reducing enzyme. In this study, we initially purified this lipid peroxide reducing protein from human blood plasma and successfully isolated apolipoprotein A1 and B100 as phosphatidylcholine hydroperoxide reducing proteins. In order to clarify the pathophysiological roles of the activity, we have developed an assay method to measure plasma phosphatidylcholine hydroperoxide reducing activity using synthetic 1-pdtoyl-2-nnoleoyl-phosphatidylchonne hydroperoxide (PLPC-OOH) as the substrate. By using this method, PLPC-OOH reducing activity was determined in the plasma of 53 normal pregnancies, 11 patients of pre-eclampsia, 18 pre-stage and 11 high-risk groups for pre-eclampsia. PLPC-OOH reducing activity was greater in the high-risk group than normal pregnancy, while lipid peroxide level was greater in the pre-eclamptic patients. The present results suggested an important role(s) for PLPC-OOH reducing activity in elimination of the plasma lipid hydroperoxide, and further involvement in the development of pre-eclampsia.