THE ANALYSIS OF THE VKH DISEASE SPECIFIC ANTIGEN - SDS INSOLUBLE PROTEIN

我们一直在分析黑素细胞的不溶性部分。我们报道了在黑素细胞中特异性表达的酪氨酸酶家族蛋白可诱导Lewis大鼠实验性Vogt Koyanagi Harada病（EVKH）。在本研究中，我们对色素大鼠进行EVKH诱导。临床上，一些眼底表现出所谓的“晚霞眼底”样外观。组织学上，色斑大鼠眼底脉络膜明显增厚、Dalen-Fucks结节及肉芽肿性炎性改变。眼部外的皮肤炎性病变和脑膜炎的迹象也发现在输注大鼠。对VKH病患者进行淋巴细胞增殖试验。VKH患者的淋巴细胞增生对抗酪氨酸酶家族蛋白衍生的肽。综上所述，VKH疾病是一种酪氨酸酶家族蛋白诱导的自身免疫性疾病。

We have been analysing the unsoluble fraction of melanocytes. We reported that tyrosinase family protein that is expressed specifically in the melanocytes can induce the experimental Vogt Koyanagi Harada disease(EVKH) in Lewis rat. In this study, we induced EVKH in pigmented rats. Clinicaly, some of the fundus shows so called 'sunset glow fundus' like appearance. histologically, markedly thickning of the choroid, Dalen-Fucks nodule and granulomataous inflammatory changes were found in the eye of the pigmented rats.Extra ocular sign of the inflammatory lesion of the skin and meningitis were also found in the pimented rats.The lymphocyte proliferation assay of the VKH disease patients was done. the lymphocytes of the VKH disease patients proliferated against the peptides derived from tyrosinase family proteins. Taken together, it is suggested that VKH disease is an autoimmune disease induced against the tyrosinase family proteins.