Pregnancy diagnosis based on the fecal oestrone sulphate concentrations using an enzymeimmunoassay in the Mare

本研究描述了一种能使母马怀孕并从配对后约100天开始监测的粪便硫酸雌酮(OS)的真酶免疫分析(EIA)。用抗原包被的方法和抗雌酮硫酸酯的单抗对甲醇提取物等份进行了EIA，得到了理想的标准曲线，分别为1ug/孔抗原、1/20000稀释度和1/1000稀释度。OS-BIA的灵敏度为5pg/ml。随着妊娠的进展，OS平均浓度逐渐升高。从交配后4个月开始，妊娠母马粪便中OS浓度显著高于未妊娠母马。未怀孕的母马可以从交配后大约4个月开始识别。

The present study describes an euzymeimmunoassay (EIA) for fecal oestrone sulphate (OS) which enables pregnancy in mares and monitored from approximately 100 days onwards following matingOestrone sulphate was extracted from fresh fecal samples in mares, by shaking in mnethanol for overnight at room temperature. Aliquots of the methanol extracts were subjected to EIA, using an antigen-coated assay format and monoclonal antibody to oestrone sulphate.The ideal standard curve was derived using lug/well antigen, 1/20,000 dilution of first antibody and 1/1,000 dilution of second antibody. The sensitivity of the OS BIA was <5pg/ml. The mean OS concentrations rose progressively as pregnancy advanced. From 4 months after mating onwards, the fecal OS concentrations of samples from pregnant mares exceeded significantly the values of non-pregnant mares. Non-pregnant mares can be identified from approximately 4 months postmating onwards.