Methodological studies of a novel physiological culture system that mimics luteal angiogenesis

卵巢周期的特征在于细胞增殖、分化和转化的重复模式，包括卵泡发育和排卵以及黄体（CL）的形成、功能和消退。近年来，人们已经确定，局部产生的血管生成因子（VEGF，FGF，IGF）通过其自分泌和旁分泌作用，在卵巢功能的不同阶段发挥重要的调节作用。我们的体内和体外研究结果表明，VEGF和FGF2是黄体血管生成的最重要的因素。最近的研究表明，一个生理相关的原代培养系统，模仿黄体血管生成的发展。该系统包含CL组织的细胞（例如内皮细胞、类固醇生成细胞、成纤维细胞、周细胞）。使用这种方法，内皮细胞在第2天开始形成簇，然后增殖并形成Threa. d样结构。在培养9天后，这些管状结构延长，伸展并形成高度组织化的复杂网络，类似于毛细血管床。使用图像分析，通过用纤连蛋白包被威尔斯孔来促进脉管系统发育。

The ovarian cycle is characterized by repeating patterns of cellular proliferation, differentiation and transformation that encompass follicular development and ovulation as well as the formation, function and regression of the corpus luteum (CL). In the last years, it is well established that locally produced angiogenic factors (VEGF, FGF, IGF) by their autocrine and paracrine effects, play an important modulatory role during different stages of ovarian function. Our in vivo and in vitro results showed that VEGF and FGF2 are the most important factors for luteal angiogenesis. The recent studies demonstrated the development of a physiologically relevant primary culture system that mimics luteal angiogenesis. This system incorporates cells of CL tissue (e.g. endothelial, steroidogenic cells, fibroblasts, pericytes). Using this approach, endothelial cells start to form cluster on day 2, which than proliferate and develop threa.d-like-stuctures. After 9 days in culture, these tube-like structures lengthen, thicken and form highly organized intricate networks resembling a capillary bed. Using image analyses, vasculature development was promoted by coating wells with fibronectin.