Study of separation based on the complexation of boron compouunds for neutron capture therapy by using zone electrophoresis.

在区带电泳上观察到的迁移是毛细管作用、电渗透流动和电泳运动的总和。研究了对硼苯丙氨酸（p-BPA）及其相关化合物在不同电泳条件下的电泳行为。比较了对双酚a、间双酚a和对硼苯基丝氨酸（p-BPS）与常用氨基酸[酪氨酸（Tyr）、苯丙氨酸（Phe）和3,4-二羟基苯丙氨酸]在酸性草酸支撑溶液中的迁移距离。p-BPA、m-BPA和p-BPS表现出不同寻常的阴离子运动。用草酸支撑液从Tyr、Phe和p-BPA的混合物中特异性分离得到p-BPA。用含氯化钠的乙二醇水溶液研究了对双酚a的区电泳行为。在任何支持溶液中均未观察到p-BPA的阴离子运动。随着溶液中氯化钠浓度的降低，电流急剧下降。对双酚a在烟酸或己三酸支持溶液中不向阴离子侧迁移。对双酚a在异柠檬酸或柠檬酸支持溶液中均有络合作用。通过电泳研究了p-BPA配合物对载体溶液ph值的稳定性。研究了不同载体（纯纤维素纸、醋酸纸、磷酸纸）对p-BPA迁移的影响。

The observed migration on zone electrophoresis is a summation of capillary action, electro-osmotic flow and electrophoretic movement. Electrophoretic behavior of p-boronophenylalanine (p-BPA) and its related compounds was studied under various electrophoresis conditions.1. The migration distances of p-BPA, m-BPA and p-boronophenylserine (p-BPS) in acidic oxalate supporting solutions were compared with those of usual amino acids [Tyrosine (Tyr), Phenylalanine (Phe) and 3,4-dihydroxy phenylalanine]. p-BPA, m-BPA and p-BPS showed unusual anionic movements. p-BPA was specifically isolated from a mixture of Tyr, Phe and p-BPA by using a oxalate supporting solution.2. Zone electrophoretic behavior of p-BPA was studied by using aqueous ethylene glycol containing sodium chloride. Anionic movement of p-BPA was not observed in any supporting solution. The electric current dropped sharply with decreasing concentration of sodium chloride in solutions.3. p-BPA did not migrate to the anion side in acotinic or trimesic acid supporting solution. The complexation of p-BPA was found in isocitric or citric acid supporting solution.4. The stability of complexes of p-BPA against the pH-values of supporting solution was studied by the electrophoresis.5. Effect of the various supports (pure cellulose paper, acetate paper, phosphate paper) on migration of p-BPA was investigated.