Immobilization of polysialic acid on the surfaces of biometerials

该项目的主要目的是通过将生物聚合物固定在生物材料的表面来产生不溶形式的聚唾液酸(PolySia)，以便使其可用于生物材料的功能。PolySia将附着在基于(生物惰性)硅胶和(可生物降解)聚磷腈聚合物的神经引导通道的表面，以及具有多种功能的二氧化硅纳米颗粒上。这些产品将在课题组的其他WPS上进行测试。如果可能，连接路线适用于各自表面的特定化学成分，如有可能，采用WP2.2和WP2.3中也使用的“点击化学”方法。此外，我们将继续表征PolySiA及其衍生物在溶液中的基本物理化学特征，特别是关于大分子及其聚集体的形状，以及晶体固体中的基本物理化学特征。光和小角X射线散射技术将被用来研究溶液中的行为，X射线衍射和模拟技术将被用来得出结晶多晶硅的结构。由此获得的关于PolySIA的结构和行为的一般信息将对其他WPS具有基本的重要性。

The main aim of this project is to generate insoluble forms of polysialic acid (polySia) by immobilizing this biopolymer to the surfaces of biomaterials, in order to make it available for biomaterials functions. PolySia wilt be attached to the surfaces of nerve guidance channels based on (bioinert) silicone and (biodegradable) polyphosphazene polymers and to silica nanoparticles with diverse functions. The products will be tested in other WPs of the research group. The attachment routes are adapted to the specific chemistry of the respective surfaces, if possible involving the "click chemistry" approach which is also used in WP2.2 and WP2.3. In addition, we will continue to characterize the basic physico-chemical characterization of polySia and its derivatives in solution, especially with regard to the shape of the macromolecules and their aggregates, and in the crystalline solid. Light and smallangle X-ray scattering techniques will be employed for studying the behaviour in solution, and X-ray diffraction and modelling techniques will be used to derive the structure of crystalline polySia. The general information on the structure and behaviour of polySia thus obtained will be of fundamental importance for other WPs.