Cardiac electrophysiology evaluation system for improved drug assessment

我们计划在二维板上复制行波，并优化获得成熟hiPSC衍生心肌细胞的最佳条件，将行波成熟心肌细胞与成体细胞进行比较。一年中取得的成就包括：1.；在平板和纳米纤维片上培养的心脏组织中都产生了行波。利用MEA可以记录心脏组织的电信号。2.通过染色、透射电镜、rnas测序等方法证实行波成熟心肌细胞的成熟度。将其成熟程度与人类成人进行了比较。3. 在日本流通学会第87届年度科学会议上发表论文2篇并作口头报告，获得青年研究员奖。

We planned to reproduce the traveling wave on 2D plate and optimize the best condition for obtain matured hiPSC derived cardiomyocytes, to compare the traveling wave matured cardiomyocytes with adult cells. The achievements in the year include: 1. The traveling wave has been generated in cardiac tissue cultured on both plate and nanofiber sheets. The electrical signal of cardiac tissue could be recorded by using MEA. 2.The maturity of traveling wave matured cardiomyocytes has been confirmed by using staining, TEM, RNAsequencing and other methods. Their maturation level has been compared with those from human adult. 3. We published 2 papers and gave an oral presentation in 87th Annual Scientific Meeting of the Japanese Circulation Society, and obtained the young investigator award.