SFB 854: Molecular Organisation of Cellular Communications within the Immune System

该项目旨在阐明调节健康和疾病免疫反应的通讯过程和网络的分子基础。为此，各个CRC854项目在细胞间和细胞内以及特定器官水平上评估免疫细胞通信的分子机制。在该项目中实施和开发了最先进的生化、遗传和成像技术，以实现科学目标。在项目的第二个资助期产生的知识和分子/遗传工具箱将为项目计划在第三个资助期进行的研究提供基础。与以前一样，研究计划分为两个研究领域：研究领域A的项目：“炎症和感染中的分子和细胞通讯”，研究细胞内和细胞间通讯过程的分子机制，重点是特定器官(脑、肝脏、肾脏、造血系统、皮肤)以及病原体或恶性肿瘤特定的背景。B研究领域“T淋巴细胞的分子和细胞调控”的项目集中于不同的信号通路及其对T细胞发育、激活和效应功能的影响。生物化学方面的深厚专业知识将与新型体内信号报告系统(生物传感器)相结合，以研究调节T细胞分化动力学或其与其他细胞的局部和系统相互作用的信号过程。这两个项目都嵌入了A区和B区，并将它们连接在一起，这是一种范式转变的结果，即大脑不再被视为免疫特权器官，与免疫系统被血脑屏障隔开。相反，现在已经确定的是，中枢神经系统和免疫系统不断地相互作用，并影响彼此的功能。因此，这两个项目解决了免疫系统和中枢神经系统之间的通信是如何受到分子调控的问题。CRC854旨在了解生理和病理生理免疫反应过程中信号处理的分子机制，并将细胞内信号机制与细胞间相互作用的动力学联系起来。为了实现这些目标，CRC854将结合当地免疫学和神经科学领域的专业知识，创造附加值。此外，CRC854已经并将进一步建立新的模型系统和方法，用于研究决定免疫激活和调节失调的分子机制。

The project aims at elucidating the molecular basis of communication processes and networks that regulate immune responses in health and disease. To this end, the individual CRC854 projects assess the molecular mechanisms of immune cell communication at the intercellular and intracellular level as well as with regard to specific organs. State of the art biochemical, genetic and imaging technologies are implemented and developed within the project to achieve the scientific goals. The knowledge and the molecular/genetic toolboxes generated during the 2nd funding period of the Project will provide the basis for the research that the project plans for the 3rd funding period. As before, the research program is divided into two research areas: Projects in Research Area A: "Molecular and cellular communication in inflammation and infection" study the molecular mechanisms of intra- and intercellular communication processes with a focus on organ-specific (brain, liver, kidney, hematopoietic system, skin), as well as pathogen- or malignancy-specific contexts. The projects of Research Area B: "Molecular and cellular regulation of T lymphocytes" focus on different signaling pathways and their impact on T cell development, activation and effector functions. Profound expertise in biochemistry will be combined with novel in vivo signaling reporter systems (biosensors) to study signaling processes regulating the dynamics of T cell differentiation or their local and systemic interactions with other cells. The TWIN projects - embedded in both Area A and Area B and connecting them - result from the paradigm shift that the brain can no longer be viewed as an immune-privileged organ, separated from the immune system by the blood-brain barrier. Instead it is now well established that the CNS and the immune system constantly interact with each other and influence each other’s functions. Therefore the TWIN projects address the question how communication between the immune system and the CNS is molecularly regulated.Taken together, CRC854 aims to understand the molecular mechanisms of signal processing during physiological and pathophysiological immune responses, and to connect intracellular signaling mechanisms with the dynamics of intercellular interactions. To achieve these goals, CRC854 will create added value by combining the local expertise in the fields of immunology and neuroscience. In addition, CRC854 did and further will establish new model systems and methodologies for the investigation of molecular mechanisms determining immune activation and dysregulation.