Core D - Immune Monitoring Core

人体免疫监测核心将表征表型和功能，体外和体内 体内特征以及对DENV感染的先天性和适应性免疫应答的分析 而关于体液免疫应答的信息， DENV将从项目1描述的感染患者的纵向研究中获得。 总之，这些经验证的测定将生成数据，以与 基因组和蛋白质组核心，并有助于具有临床价值的DENV签名。我们 预计质谱技术和样品微型化的进步 程序可能会增加全球和功能分析的敏感性， 这些分析所需的样本量，允许扩展先天免疫能力 对受感染和接种疫苗的患者进行的研究， 与DENV感染后的临床状态、疫苗结果或DC致病性相关。 这些测定法构成了未来开发新的免疫增强剂的基础。 促进保护性免疫反应发展的策略。

The Human Immune Monitoring Core will characterize phenotypic and functional, ex vivo and in vivo signature as well as analyses of innate and adaptive immune responses to DENV infection and challenges at various time points whereas information on humoral immune responses to DENV will be obtained from the longitudinal studies of infected patients described project 1. Together, these validated assays will generate data to be integrated with results from the genomic and proteomic cores, and contribute to a DENV signature with clinical value. We anticipate that advances in mass spectrometry technologies and sample miniturization procedures may increase the sensitivity of global and functional analyses and may reduce the amount of sample required for these analyses allowing to extend innate immune competence studies to infected and vaccinated patients further refining the identification of a signature correlated with clinical status upon DENV infection, vaccine outcome or DC pathogenicity. These assays comprise the groundwork for future development of new immune enhancing strategies to promote the development of a protective immune response.