Disclipine A - Analytical Track 4: Chemistry Capability/Capacity Development

摘要 项目四：能力扩展：德克萨斯州分离株的全基因组测序 动物性食品 德克萨斯州化学家办公室(OTSC)有能力筛选和/或确认以下事项 微生物：沙门氏菌属。动物性食品、动物性食品配料、生物固体、人类食品； 动物性食品、动物性食品中产志贺样毒素的大肠杆菌(STEC)O157：H7和非O157 配料和人类食品.生牛奶、动物性食品和动物性食品中的单核细胞增多性李斯特菌 配料，人类食品；原料奶中的SEB，布鲁氏菌。原料奶中的抗原，蓖麻毒素通过 Tetracore酶联免疫吸附试验，志贺氏菌。在人类食物中，炭疽杆菌在人类食物中， 食品中葡萄球菌肠毒素、T-2毒素和α-氨基甲酸甲酯的LCMMS分析。这个 MicroLab接受了炭疽芽孢杆菌和耶尔森氏菌筛选方法学的BSL3培训 2018年在马里兰州德特里克堡的USAMRIID设施中发现鼠疫病毒，并正在推进建立 方法(筛选和确认)在OTSC设施。OTSC全基因组测序 能力开发项目包括：1)购买MiSeq测序平台和 必要的工具，2)完成并通过能力评估，3)扩大 OTSC ISO/IEC 17025.2017范围包括基因组测序。作为一种结果， 能力扩展：德克萨斯州动物性食品分离株全基因组测序项目， OTSC将在实验室内部使用MiSeq开始对李斯特菌和 沙门氏菌DNA，这将加强疾病监测和流行病学。

Abstract PROJECT FOUR: Capacity Expansion: Texas Whole Genome Sequencing of Isolates from Animal Food Office of the Texas State Chemist (OTSC) has the ability to screen and/or confirm the following organisms: Salmonella spp. in animal food, animal food ingredients, biosolids, human food; Shiga like toxin producing E.coli (STEC) O157:H7 and non-O157 in animal food, animal food ingredients and human food; Listeria monocytogenes in raw milk, animal food and animal food ingredients, human food; SEB in raw milk, Brucella spp. antigen in raw milk, ricin toxin via Tetracore ELISA, Shigella spp. in human food, Bacillus anthracis sterne in human food, Staphylococcal enterotoxins in human food, T-2 toxin and alpha- amanitin by LCMSMS. The microlab received BSL3 training in screening methodologies of Bacillus anthracis and Yersinia pestis at USAMRIID facility, Ft Detrick, MD in 2018 and are moving forward to establish this method (screening and confirmatory) at OTSC facility. The OTSC whole genome sequencing capability development project includes: 1) purchasing MiSeq sequencing platform and necessary instruments, 2) completing and passing competency assessment, 3) expanding the OTSC ISO/IEC 17025.2017 scope to include genome sequencing. As an outcome of the Capacity Expansion: Texas Whole Genome Sequencing of Isolates from Animal Food project, OTSC will begin DNA sequencing using a MiSeq internally within the lab for Listeria and Salmonella DNA, which will strengthen disease surveillance and epidemiology.