Cytek 3-laser Aurora

项目摘要/摘要 西弗吉尼亚大学(WVU)流式细胞仪和单细胞核心设备(FCSCCF)是一种集中式 机构共享的研究资源设施，为西弗吉尼亚大学和其他地区的研究界提供服务 机构。西弗吉尼亚大学FCSCCF按照运营和管理方面的最佳做法运作 用于机构共享的研究设施。服务是由用户的NIH资助项目的需求驱动的 在4个主要领域的生物医学研究：1)由研究人员发起的赠款(R01和R21)，2)来自 国家普通医学科学研究所(NIGMS)生物医学研究卓越中心 P20 GM121322和P20 GM109098)，3)NIGMS支持的临床和翻译科学研究所(CTSI， U54 GM104942)和4)NIGMS支持的IDEA卓越生物医学研究网络(INBRE P20 GM103434)计划。目前，使用西弗吉尼亚大学FCSCCF的研究人员可以使用两种流式细胞仪： 1)一台4激光器Becton Dickinson(BD)LSRFortessa分析流式细胞仪，由NIH S10拨款购买 (2013年授予)和2)4激光BD FACSAria III细胞分选器，2017年用WV的资金购买 CTSI。LSRFortessa的高使用率导致一些研究人员在他们的样本上分析了更多 昂贵和较慢的FACSAria III细胞分选机，这增加了他们的研究时间和成本。2019年， 分析样品的计费使用是可访问用户时间(AUT)的85.6%(1189.4小时(71.5%AUT)) 在LSRFortessa上和235.8小时(14.2%AUT)在FACSAria III上)。在过去的一年里，有两个用户 需要检测超过LSRFortessa在一次 样本，并已将样本送往弗吉尼亚大学的流式细胞仪中心进行分析 赛特克·奥罗拉。通过将样本发送到另一家机构，研究人员会招致更高的成本和更长的滞后时间 在样本收集和数据分析之间。此外，几位西弗吉尼亚大学FCSCCF用户研究稀有细胞 当用户被迫分析FACSAria III上的样本时，这一点尤其具有挑战性。 在评估了几种分析流式细胞仪后，我们确定Cytek 3-LASER Aurora是最好的 功能组合，以满足WVU FCSCCF用户当前和预期的需求。增加了 Cytek流式细胞仪将通过增加对尖端技术的访问来支持我们由NIH资助的研究人员 技术，减少等待时间和成本，从而提高竞争力。

PROJECT SUMMARY/ABSTRACT The West Virginia University (WVU) Flow Cytometry & Single Cell Core Facility (FCSCCF) is a centralized institutional shared research resource facility that serves the research community at WVU and other regional institutions. The WVU FCSCCF operates in accordance with best practices in operations and management for institutional shared research facilities. Services are driven by the needs of the users’ NIH-funded projects in biomedical research in 4 primary areas:1) investigator-initiated grants (R01 and R21), 2) grants from the National Institute of General Medical Sciences (NIGMS) Center of Biomedical Research Excellence (CoBRE P20 GM121322 and P20 GM109098), 3) NIGMS supported Clinical and Translational Science Institute (CTSI, U54 GM104942), and 4) NIGMS supported Idea Network for Biomedical Research Excellence (INBRE P20 GM103434) programs. Currently, two flow cytometers are available to researchers using the WVU FCSCCF: 1) a 4-laser Becton Dickinson (BD) LSRFortessa analytic flow cytometer, purchased with an NIH S10 grant (awarded in 2013) and 2) a 4-laser BD FACSAria III cell sorter, purchased in 2017 with funds from the WV CTSI. High usage of the LSRFortessa has caused some researchers to analyze their samples on the more expensive and slower FACSAria III cell sorter, which increases the time and cost of their research. In 2019, billed usage for analytical samples was 85.6% of the accessible users time (AUT) (1189.4 hr (71.5% AUT) on the LSRFortessa and 235.8 hr (14.2% AUT) on the FACS Aria III). In the past year, two users have needed to detect more than the maximum of 17 fluorochromes that the LSRFortessa can detect in a single sample and have been sending samples to the University of Virginia’s flow cytometry core for analysis on a Cytek Aurora. By sending samples to another institution, researchers incur a higher cost and longer lag times between sample collection and data analysis. Additionally, several WVU FCSCCF users study rare cell populations, which is particularly challenging when users are forced to analyze samples on the FACSAria III. After evaluating several analytical flow cytometers, we determined that the Cytek 3-laser Aurora has the best combination of features to meet the WVU FCSCCF users’ current and anticipated needs. Addition of the Cytek flow cytometer will support our NIH-funded investigators by increasing access to cutting edge technology and reducing wait time and costs, thereby increasing competitiveness.