"Acquisition of a Multi-functional Cryo Scanning Electron Microscope (SEM)"

项目摘要 获取多功能低温扫描电子显微镜： 马里兰州巴尔的摩大学（UMB）的电子显微镜核心成像设备（EMCIF） 申请资金购买集成的低温扫描电子显微镜（SEM），Apreo S LoVac， 赛默飞世尔科技（Thermo Fisher Scientific）（原FEI公司）。这种多功能仪器与自动化 导航和成像分析软件，能够在室内对精细的生物标本进行成像 温度和冷冻水合状态。配套程序的目的是关联光学显微镜 通过电子显微镜（EM）获得具有超微结构细节的信号，并扫描连续切片，从而 允许对组织样本进行3D成像。11名NIH资助的研究人员代表8项研究 研究广泛的重要生物医学研究课题的团体将成为主要用户。研究 这些主要使用者的领域包括癌症、肥胖症、糖尿病、肾脏疾病、传染病、神经系统疾病、 退化、听力损失、骨/肌腱发育、药物开发、医疗器械设计，以及 诊断和治疗策略，都将受益于新仪器的能力。五 额外的小用户将受益于在室温和低温下增强的分辨率， 研究用于疫苗研究的生物膜和用于纳米技术医疗的水凝胶和微球 设备和治疗策略。要求的仪器将放置在当前的EMCIF内， 一个有组织的核心设施，提供最先进的电磁仪器和培训，超过150 研究人员、实习生和工作人员每年。强有力的机构支持为主任提供持续支持 以及员工工资、仪器服务/维护和其他EMCIF运营成本、新信息 存储/计算能力，并为购买新仪器提供10万美元的捐款。应该 这项赠款被授予，这种独特的和多功能的低温SEM系统的最终影响将超越 本研究计划书中所列的研究项目。总的来说，这一新能力将补充现有的成像UMB 能力，并扩大在UMB和区域机构的生物医学研究的能力。

PROJECT SUMMARY Acquisition of a multi-functional cryo scanning electron microscope: The Electron Microscopy Core Imaging Facility (EMCIF) of the University of Maryland Baltimore (UMB) requests funds to acquire an integrated cryo scanning electron microscope (SEM), the Apreo S LoVac from Thermo Fisher Scientific (formerly FEI Company). This multifunctional instrument is bundled with automated navigation and imaging analysis software and is capable of imaging delicate biological specimens at room temperature and in a frozen hydrated state. Companion programs are designed to correlate light microscopy signal with ultrastructural detail obtained by electron microscopy (EM), and to scan serial sections, thereby allowing imaging of a tissue specimen in 3-D. Eleven NIH funded investigators representing eight research groups studying a wide range of important biomedical research topics will be Major Users. The research domains of these Major users include cancer, obesity, diabetes, kidney diseases, infectious diseases, neural degeneration, hearing loss, bone/tendon development, drug development, medical device design, and diagnostic and therapeutic strategies that will all benefit from the capabilities of the new instrument. Five additional Minor Users will benefit from the enhanced resolution at both room and cryo temperatures to examine biofilms for vaccine research and hydrogels and microspheres for nanotechnology-based medical devices and therapeutic strategies. The requested instrument will be placed within the current EMCIF, a well- organized core facility that provides access to state-of-the-art EM instrumentation and training to over 150 researchers, trainees and staff each year. Strong institutional support provides ongoing support for Director and staff salaries, instrument service/maintenance and other EMCIF operating costs, new information storage/computing capacity, and a $100,000 contribution toward the purchase of the new instrument. Should this grant be awarded, the ultimate impact of this unique and versatile cryo SEM system will extend beyond the research projects listed in this proposal. Overall, this new capacity will complement existing imaging UMB capabilities and expand the capacity for biomedical research at UMB and regional institutions.