Orbitrap Exploris 480 Basic System

项目摘要 我们提出了获得热科学™Orbitrap™Exploris480质量的建议 该光谱仪将安装在阿尔伯特·爱因斯坦医学院的蛋白质组学核心中。这 仪器对于我们NIH资助的研究人员进行蛋白质组学实验至关重要。特定的 本提案中提出的将从这一文书中获得巨大好处的项目包括研究 传染病(单纯疱疹病毒、SARS-CoV2、弓形虫、微孢子虫等)、癌症 骨髓增生异常综合征，癌前和癌症干细胞的分子调控和靶向 造血和白血病)、衰老(衰老过程中的自噬、神经变性)、心血管 疾病和基础细胞生物学(肥胖、细胞死亡机制、染色质、细胞信号)。这个 爱因斯坦蛋白质组学核心需要这台仪器来取代热科学™轨道捕捉™VELOS。这个 Orbitrap Velos于2010年被收购，它一直是Core唯一安装了蛋白质组学的工具，但它 现在需要更换，因为到2020年底，它将不再由供应商提供服务。实验室 蛋白质组学设施的空间最近已经完全翻新，Simone Sidoli博士受雇于 领导爱因斯坦的蛋白质组学研究。去年，蛋白质组实验室检测了2500多个来自 大约50个不同调查人员的实验室。爱因斯坦蛋白质组学核心也是一个重要的支持 为爱因斯坦癌症中心、内森休克中心提供服务，以求卓越的衰老生物学研究 (E-NSC)和ERC-CFAR爱因斯坦-洛克菲勒-纽约州立大学艾滋病研究中心。

Project Summary We present our proposal for the acquisition of a Thermo Scientific™ Orbitrap™ Exploris 480 mass spectrometer that would be installed in the Proteomics Core at the Albert Einstein College of Medicine. This instrument is critical for the performance of proteomics experiments by our NIH funded investigators. Specific projects presented in this proposal that will benefit enormously from this instrument include research on infectious diseases (herpes simplex virus, SARS-CoV2, Toxoplasma gondii, microsporidia, etc.), cancer (myelodysplastic syndrome, molecular regulation and targeting of pre-cancerous and cancer stem cells in hematopoiesis and leukemogenesis), aging (autophagy during aging, neurodegeneration), cardiovascular diseases and fundamental cell biology (obesity, mechanisms of cell death, chromatin, cell signaling). The Einstein Proteomics Core requires this instrument to replace a Thermo Scientific™ Orbitrap™ Velos. The Orbitrap Velos was acquired in 2010, and it has been the only proteomics-fitted instrument for the Core, but it now needs to be replaced as by the end of 2020 it will no longer be serviced by the vendor. The laboratory space of the proteomics facility has recently been completely renovated and Dr. Simone Sidoli was hired to lead proteomics research at Einstein. In the last year, the proteomics laboratory ran over 2,500 samples from approximately 50 different investigators' laboratories. The Einstein Proteomics Core is also a critical support service for the Einstein Cancer Center, the Nathan Shock Center for excellence on Biology of Aging Research (E-NSC) and the ERC-CFAR Einstein-Rockefeller-CUNY Center for AIDS research.