In Vivo Fluorescence and Bioluminescence Imager with CT

项目总结 在整个动物上进行生物发光和荧光活体成像已经成为一种 生物医学研究人员在许多领域日益重要和必要的工具，包括 癌症、神经生物学、传染病和炎症。活体成像有助于更好地 了解疾病病理、疾病监测和药物开发。Perkin Elmer‘s IVIS SpectrumCT测量生物发光、荧光、2D和3D成像能力 并包括集成的低剂量和超快CT成像。许多研究项目 本申请中描述的是翻译性质的，并将导致新的治疗方法或 更好地了解疾病状态。我们目前的系统购买于2005年，并于 并已变得不可靠，导致仪器严重停机。我们中的许多人 进行小鼠纵向实验的研究人员损失了时间、金钱、数据和精力 由于我们目前的IVIS Xenogen出乎意料地不可用。我们有近55名用户 现有系统来自马里兰大学巴尔的摩分校(UMB)内的学校和各种 卓越的中心。我们已经确定了一个由20名NIH资助的调查人员组成的小组，其中36名是NIH 和其他联邦拨款，其个别项目将直接和实质性地受益 通过继续获得可靠的、最先进的仪器来进行生物发光 以及整个动物的荧光成像。该机构对以下方面的重大承诺 进行了安装和持续的长期运行，以获得最新状态 具有内置CT系统的ART生物发光/荧光系统用于3D层析成像 申请。该文书的管理和业务结构已经到位。 拥有一支优秀的技术团队，努力为最终用户提供优质的服务。

PROJECT SUMMARY Bioluminescent and fluorescent in vivo imaging on whole animal has become an increasingly important and necessary tool for biomedical researchers in many fields including cancer, neurobiology, infectious diseases and inflammation. In vivo imaging facilitates a better understanding of disease pathology, disease monitoring, and drug development. Perkin Elmer’s IVIS SpectrumCT measures bioluminescence, fluorescence, 2D and 3D imaging capabilities and included an integrated low-dose and ultra-fast CT imaging. Many of the research projects described in this application are translational in nature and will lead to new therapeutics or better understanding of disease states. Our current system purchased in 2005 and updated in 2012 and has become unreliable leading to significant downtime of the instrument. Many of our investigator who conduct longitudinal mice experiments have lost time, money, data and effort due to unexpected unavailability of our current IVIS Xenogen. We have nearly 55 users of the existing system from schools within the University of Maryland Baltimore (UMB) and various centers of excellence. We have identified a group of 20 NIH-funded investigators with 36 NIH and other federal grants whose individual projects would be directly and substantially benefitted by having continued access to a reliable, state-of-the-art instrument to conduct bioluminescence and fluorescence imaging in whole animals. Significant commitment by the institution towards installation and sustained long-term operations has been made in order obtain the state-of-the- art bioluminescence/fluorescence system with a built-in CT system for 3D tomography applications. The management and operational structure for this instrument is already in place with an outstanding technical team that strives to provide excellent service to the end users.