Acquisition of A Microfluidic Chip-Based System for Cluster Sorting and Dispensing

项目总结/摘要 该提案要求为基于微流控芯片的系统提供资金，该系统将实现集群分类， 配药。这将是斯坦福大学核心设施提供的第一个系统。系统将填充 对组织微环境中的细胞进行分选的既定但未满足的需求。它将安装在外地资产管制系统 中心位于研究所干细胞生物学和再生医学，一个核心设施开放给所有 斯坦福大学的研究人员。该文书的主要和次要用户都有能力应用这一点， 仪器沿着下一代组学技术，用于 脆弱的细胞及其附着的微环境的技术，如体内移植和体外 培养，活体成像，解离簇的单细胞RNA测序，CyTOF分析，共聚焦显微镜， 显微镜下的生活集群，蛋白质组学分析使用质谱仪，以满足他们的NIH资助的目标， 研究计划，包括干细胞生物学，血管生物学，癌症生物学， 免疫学 干细胞生物学 ·表征造血干细胞的骨髓小生境，神经干细胞的植入机制， 干细胞 ·分析非处方药如何改变小鼠的免疫微环境 COVID-19诱导的ARDS模型 血管生物学 ·了解不同细胞类型和病毒抗原（包括流感和SARS）在复杂的 动脉粥样硬化病变 癌症生物学 ·了解肿瘤微环境 免疫学 ·了解驻留和浸润免疫和皮肤细胞如何影响伤口愈合 ·探究胸腺细胞发育背后的机制 该系统将被放置在一个完善的，非常成功的核心设施开放给所有研究人员在斯坦福大学 保险在这台仪器的生命周期中，它将被来自数十个实验室的数百名研究人员使用， 支持类似的优秀研究，以更好地了解和战胜疾病。

Project Summary/Abstract This proposal requests funding for a microfluidic chip-based system that will enable cluster sorting and dispensing. This will be the first system made available at a Core facility at Stanford University. The system will fill an established, unmet need for sorting cells in their tissue microenvironment. It will be installed at the FACS Center located at the Institute for Stem Cell Biology and Regenerative Medicine, a Core facility open to all researchers at Stanford University. The major and minor users of the instrument are positioned to apply this instrument along with next generation -omic technologies for molecular and functional characterization of delicate cells and their attached microenvironments with techniques such as in vivo transplantation and in vitro culture, intra vital imaging, single cell RNA sequencing of dissociated clusters, CyTOF analysis, confocal microscopy of living clusters, proteomic analysis using mass spectrometry, to meet the goals of their NIH-funded research programs that include investigations in stem cell biology, vascular biology, cancer biology, and immunology. Stem cell biology • Characterizing the bone marrow niches of hematopoietic stem cells, mechanisms of engraftment of neural stem cells • Analyze how over-the-counter medications can change the immune microenvironment in a mouse model of COVID-19 induced ARDS Vascular biology • Understanding the interplay of different cell types and viral antigens including flu and SARS in complex atherosclerotic lesions Cancer Biology • Understanding tumor micro-environments Immunology • Understanding how resident and infiltrating immune and skin cells affect wound healing • Interrogating the mechanisms behind thymocyte development The system will be placed in a well-established, highly successful core facility open to all researchers at Stanford insures. Over the life of this instrument, it will be used by hundreds of researchers from many tens of labs to support similarly excellent research to better understand and combat disease.