Replacement of an outdated 600 MHz NMR CryoProbe system

NIH共享仪器拨款(SIG)计划，PAR-20-113 项目摘要/摘要： 维持和扩大强场生物分子核磁共振研究计划 马里兰大学园区分校(UMCP)，我们正在申请SIG补助金，以取代 布鲁克600 MHz核磁共振的老化低温探头系统 生物分子核磁共振设备的光谱仪。我们建议购买的组件 是1)第5代低温平台，2)BNSL/a氮气再液化助剂，3)a CRYO-TCI H&F探头和BBFO室温探头，4)样品 系统。 2002年安装的配备了Cryo-TXI探头的600 MHz核磁共振是第一个 UMCP园区的现场核磁共振。光谱仪是研究化学和化学物质的有力工具。 生物分子结构、分子动力学和亲和力，以及分析 由于低温探头的高灵敏度，低浓度的化合物。因为它的 安装后，该光谱仪已每天为研究生和教职员工服务 校园内各个领域的成员，以及定期来自邻近学术机构的用户 和政府研究机构。它已经成为几个人的重要工具 NIH资助项目的首席调查人员(PI)。然而，光谱仪有 由于其老化的频繁维修，近年来开始有越来越长的停机时间 CryoPlatform和Cryo-TXI探针，扰乱了用户的核磁共振研究活动。 我们认为当务之急是解决低温探测器系统的可靠性问题。 光谱仪，以确保系统的可持续性和性能。这项建议 因为更换得到了NIH资助的PIs以及其他教职员工的大力支持 在UMCP。SIG赠款将提供一个拯救老化仪器的机会，因为 以及提高仪器的性能和能力。如果获奖，UP- 最新的仪器将为UMCP核磁共振社区带来以下好处：(1)确保 美国国立卫生研究院资助的几个研究项目的进展。(2)激发新的生物分子 核磁共振研究兴趣。(三)提高生产率和效益。(4)提供亲身实践 对研究生和博士后的学习和工作经验(5) 降低液氮消耗/成本。

NIH Shared Instrumentation Grant (SIG) Program, PAR-20-113 Project Summary/Abstract: To sustain and expand the strong high field Biomolecular NMR research programs at the University of Maryland, College Park (UMCP), we are applying for a SIG grant to replace an aging Cryoprobe system of a Bruker 600 MHz Nuclear Magnetic Resonance (NMR) spectrometer at the Biomolecular NMR Facility. The components we propose to purchase are 1) a 5th generation CryoPlatform, 2) BNSL/a Nitrogen re-liquefication accessary, 3) a Cryo-TCI H&F probe, together with a BBFO room temperature probe, 4) a SampleCase system. The 600 MHz NMR equipped with a Cryo-TXI probe, installed in 2002, is the first high field NMR on UMCP campus. The spectrometer is a powerful tool to study chemical and biomolecular structures, molecular dynamics and affinities, as well as to analyze compounds in low concentration due to the Cryoprobe’s high sensitivity. Since its installation, the spectrometer has been daily serving graduate students and faculty members in various fields on campus, and periodically users from neighboring academic and government research institutes. It has been an essential instrument to several principal investigators (PIs) with NIH funded programs. However, the spectrometer has started to have increasing down time in recent years due to frequent repairs of its aging CryoPlatform and Cryo-TXI probe, disrupting NMR research activities of users. We believe it is urgent to address the reliability issue of the Cryoprobe system of the spectrometer to ensure the sustainability and performance of the system. The proposal for replacement is strongly supported by NIH funded PIs as well as other faculty members at UMCP. The SIG grant would provide an opportunity to rescue the aging instrument as well as to enhance the performance and capability of the instrument. If awarded, the up- to-date instrument will bring following benefits to UMCP NMR community: (1) Ensuring the advance of several NIH funded research programs. (2) Inspiring new Biomolecular NMR research interests. (3) Improving productivity and efficiency. (4) Providing hands-on learning and working experience to graduate students and postdocs (5) Drastically reducing liquid nitrogen consumption/cost.