Histology, Microscopy and Image Analysis Core

项目总结 组织学、显微镜和图像分析核心设计用于满足 有前途的初级研究人员、试点项目研究人员和资助的糖尿病研究人员 通过利用最先进的技术帮助他们研究糖尿病人和动物组织 组织学和显微技术，以及协助和提供 体外细胞显微镜分析所需的各种类型的显微镜。组织 准备工作需要专门的实验室和设备，而最先进的显微镜 超出了大多数实验室的预算。HMIA核心的目标有两个。一是 集中组织学准备并提供获得专门设备的途径 准备和切割组织。第二个目标是维护和提供有关 各种不同类型的最新显微镜和定量图像分析 软件。核心将提供组织处理、包埋、切片和组织化学 石蜡包埋和冷冻保存的组织制剂的固定载玻片的染色。 此外，核心将提供高质量的最先进的仪器和专业知识，以 调查人员使用明亮的视场、相位获得出版物质量的显微图像 对比度、Nomarski DIC显微镜、荧光显微镜、共聚焦和多光子 显微镜、激光捕获显微解剖显微镜和活细胞成像显微镜。至 补充这个核心的显微镜组件，通过各种帮助和培训 提供了定量的图像分析工具。核心使用的注册、计费和跟踪就是全部 使用iLabs软件完成。核心占地500平方英尺，位于Biomedical的5楼 组织学功能科学大楼，生物医学二楼150平方英尺 成像功能研究大楼。一份商业计划已经到位，这样到年底 科布雷第三阶段的核心财务将是稳定的，并准备好融入OUHSC 研究核心设施网络。

PROJECT SUMMARY The Histology, Microscopy and Image Analysis Core is designed to meet the needs of Promising Junior Investigators, Pilot Project Researchers, and Funded Diabetes Investigators by assisting them in studying diabetic human and animal tissues utilizing state-of-the-art histologic and microscopic technologies, as well as assisting them with and providing them with a variety of types of microscopy required for in vitro cellular microscopic analysis. Tissue preparation requires a specialized laboratory and equipment, while state-of-the-art microscopes are outside the budget of most laboratories. The objective of the HMIA Core is twofold. One is to centralize histological preparation of tissues and provide access to specialized equipment for preparing and cutting tissues. The second is to maintain and provide training on and access to a variety of different types of state-of-the-art microscopes and to quantitative image analysis software. The core will provide tissue processing, embedding, sectioning, and histochemical staining of mounted slides, for both paraffin embedded and cryo-preserved tissue preparations. In addition, the core will provide quality state-of-the-art instrumentation and expertise for investigators to obtain microscopic images of publication quality using bright field, phase contrast, and Nomarski DIC microscopy, epifluorescence microscopy, confocal and multi-photon microscopy, laser capture microdissection microscopy, and live-cell imaging microscopy. To complement the microscopy component of this Core, assistance and training with a variety of quantitative image analysis tools is provided. The signup, billing, and tracking of Core use is all done using iLabs software. The Core comprises 500 sq ft on the 5th floor of the Biomedical Sciences Building for the histology functions and 150 sq ft on the 2nd floor of the Biomedical Research Building for imaging functions. A business plan is in place so that by the end of the COBRE Phase III the core will be financially stable and ready to integrate into the OUHSC Research Core Facilities network.