A Research Resource for Ultra-sensitive and High Throughput Proteomics - Driving Biomedical Projects

项目摘要-推动生物医学项目 蛋白质组学研究资源更新的目标是通过以下方式广泛影响生物医学研究： 提供从更小的样品中获得更高质量的蛋白质组学数据的能力， 进行更全面的定量测量，提高低丰度 组分，如有问题的肽异构体和翻译后修饰，并使大的 增加测量吞吐量。在取得这些进展的同时，还将努力确保广泛和 持续获得新的能力，使它们继续对生物医学研究产生重大影响。到 为了帮助实现这些目标，我们已经确定了九个初步的驱动生物医学项目（DBP）。这些都 被选中的三个技术研究和开发（TR&D）项目都集中在开发 解决现实世界的问题，并作为更广泛的社区如何使用新的 技术在自己的研究项目中。每个DBP都将作为新技术初始应用的试验台。 技术和社区参与的早期阶段。九个DBPs在地理上代表 分散的合作者，从事广泛的生物医学相关的研究课题，其工作可以 新技术的应用将大大提高。DBPs将大大受益于所有 三个资源TR& D包括以下能力：操作和处理小得多的样品 (TR&D 1），以更高的通量（TR&D 2）从这些样品中提供广泛和定量的蛋白质组学数据， 并使生物信息学分析的结果数据以及生物见解的产生 (TR&D 3）。在与研发工作的配合下，DBPs还将为 确定技术研究与开发活动的优先次序，将促进问题的解决，并提供所需的挑战， 推动技术发展。它们还将推动各组织内部开发的能力的整合， 三个资源TR& D。在这一更新过程中，我们的进步将逐步使越来越多的 挑战DBPs例如，获得定量高通量蛋白质组学测量的能力， 单细胞水平，将扩大蛋白质组学的效用，为全新的研究领域。这种戏剧性 能力的进步将首先应用于几个现有的以及新的DBP中。这些项目和 他们的成功将支持我们在更广泛的社区内教育和提高认识的努力 我们的新能力。结合资源社区参与活动的其他方面， DBPs将有助于确保它们最广泛和最持久地提供给研究界。

Project Summary – Driving Biomedical Projects The goal of this renewal of the Proteomics Research Resource is to broadly impact biomedical research by providing the capabilities to obtain higher quality proteomics data from much smaller samples, together with producing more comprehensive quantitative measurements, improving coverage of low abundance components, such as problematic peptide isomers and post translational modifications, and enabling large increases in measurement throughput. These advances will be made in tandem with efforts to assure broad and sustained access to the new capabilities so they will continue to have high impact on biomedical research. To assist in achieving these goals, we have identified nine initial Driving Biomedical Projects (DBPs). These have been selected to both focus the three Technology Research and Development (TR&D) projects on developing solutions to real-world problems and to serve as examples to the wider community on how to use the new technologies in their own research projects. Each DBP will serve as a test-bed for the initial application of new technologies and the earliest stages of community engagement. The nine DBPs represent geographically dispersed collaborators, working on a wide range of biomedically-relevant research topics, and whose work can be greatly enhanced by the application of the new technologies. The DBPs will significantly benefit from all three of the Resource TR&Ds including the capabilities to: manipulate and process much smaller samples (TR&D 1), provide broad and quantitative proteomics data from such samples at higher throughput (TR&D 2), and enable bioinformatics analysis of the resulting data as well as the generation of biological insights (TR&D 3). Working in concert with the TR&D efforts, the DBPs will also provide key impetus for the prioritization of the TR&D activities, will stimulate problem solving, and provide the challenges needed for driving technology developments. They will also drive the integration of the capabilities developed within each of the three Resource TR&Ds. In the course of this renewal, our advances will progressively enable increasingly challenging DBPs. For example, the ability to obtain quantitative high throughput proteomics measurements at the single cell level, would expand the utility of proteomics for entirely new areas of research. Such dramatic advances in capabilities will be initially applied within several current as well as new DBPs. These projects and their success will support our efforts to both educate and increase the awareness within the broader community of our new capabilities. In conjunction with other aspects of the Resource community engagement activities, the DBPs will help assure their broadest and most sustained availability to the research community.