Core B: Protein Expression Core

用于评估设计的新免疫原的生化、结构和免疫实验 并选择诱导广谱中和抗体将需要表达和纯化 大量的蛋白质，包括HIV包膜蛋白和抗体。这种蛋白质 Expression Core将为该计划项目提供纯化的蛋白质，这将促进 确定免疫原和定义免疫策略的目标，以引起强烈和 有针对性的免疫反应。从免疫小鼠中分离出的抗体(项目1)将是 表达和纯化的结合和生化特性以及结构 研究(项目2)。环境蛋白，包括单体gp120s和天然类环境gp140三聚体 (SOSIP.664构造)，将在项目1中表达用于免疫，结合研究 项目1和2，以及项目2中的结构/生化分析。核心B将使用各种 表达系统对产生不同糖基化程度的Env蛋白的影响 糖基化形式对产生的免疫反应的影响。数量众多的不同 抗体和HIV蛋白的表达和纯化为这个计划需要一个专门的 蛋白质表达核心。

Biochemical, structural, and immunization experiments to evaluate new immunogens designed and selected to induce broadly neutralizing antibodies will require expression and purification of large numbers of proteins, including HIV envelope proteins and antibodies. The Protein Expression Core will provide purified proteins for this program project, which will facilitate the goals of identifying immunogens and defining immunization strategies that elicit a strong and targeted immune response. Antibodies isolated from immunized mice (Project 1) will be expressed and purified for binding and biochemical characterization as well as for structural studies (Project 2). Env proteins, both monomeric gp120s and native-like Env gp140 trimers (SOSIP.664 constructs), will be expressed for immunizations in Project 1, binding studies in Projects 1 and 2, and structural/biochemical analyses in Project 2. Core B will use various expression systems to produce Env proteins with different glycosylation to determine the effects of glycosylation form on the generated immune response. The large number of different antibodies and HIV proteins to be expressed and purified for this program requires a dedicated protein expression core.