Integrated Cancer Genomics Core

摘要整合的癌症基因组学共享资源 集成癌症基因组学共享资源(ICG)致力于提供最先进的 随着这些技术的发展，为杜克癌症研究所(DCI)的研究人员提供仪器和方案支持 随着时间的推移。ICG扩展以满足包括微生物组、表观遗传学和增加单项的DCI需求 由三个机构指定的核心设施提供的细胞服务：测序和基因组技术 最近建立的微生物组共享资源(MSR)和分子基因组学核心(SGT) (MGC)。通过为DCI成员统一这些现有资源，ICG实现了其提供一站式服务的目标 访问当代癌症中使用的所有主要研究方案和仪器平台 基因组学研究，包括基因组学、转录组学、微生物研究和表观遗传学。 十多年来，ICG一直保持着提供最新、最先进的基因组和 向DCI成员提供转录服务。ICG包括由三个杜克学派提供的服务 医药(SOM)核心设施。测序与基因组技术(SGT)与微生物组共享 资源(MSR)在杜克基因组学和计算生物学中心内提供服务； 分子基因组学核心(MGC)在杜克分子生理学研究所内提供服务。ICG 统一校园内的所有癌症基因组技术，提供对所有主要研究的一站式访问 当代癌症基因组研究中使用的协议和仪器平台。ICG支持 通过提供专家咨询、项目管理和培训，从DCI调查员那里获得广泛的项目 为了方便获取包括SNP发现、染色质修饰图谱、单细胞 测序，在几个尺度上测量mRNA水平(单基因、癌症小组、整个转录组)， 测序外显子组、鉴定DNA甲基化、微生物组图谱和绘制转录因子结合图 网站。通过提供全方位的技术平台，ICG允许调查人员选择最佳 为他们的癌症相关项目提供解决方案，并协助调查人员进行数据质量控制、版本控制、统计 对所有这些服务进行分析和传播。此外，ICG与DCI调查人员合作探索 并建立促进癌症研究进展的新技术。 2018年，ICG共享资源为290名调查员提供了服务，其中29%是DCI成员， 在所有8个DCI研究项目中，占总使用量的25%。DCI对此共享资源的使用 在项目所涉期间，成员撰写了216份出版物，其中80份刊登在影响较大的期刊上。这个 共享资源主要在回收成本的基础上运作，机构支助部分 运营成本和仪器采购。来自癌症中心支持赠款的支持使ICG能够提供 与DCI成员进行磋商，协助准备赠款和手稿，以及安排优先事项。用户 其他活动的费用遵循医学院的指导方针。

ABSTRACT– INTEGRATED CANCER GENOMICS SHARED RESOURCE The Integrated Cancer Genomics shared resource (ICG) is committed to providing state-of-the-art instrumentation and protocol support to Duke Cancer Institute (DCI) researchers as these technologies evolve over time. The ICG expanded to meet DCI needs that encompass microbiome, epigenetic and increase single cell services provided by three institutionally designated core facilities: Sequencing and Genomic Technologies Core (SGT), the recently established Microbiome Shared Resource (MSR), and the Molecular Genomics Core (MGC). By unifying these existing resources for DCI members, the ICG meets its objective to provide one-stop access to all of the major research protocols and instrumentation platforms used in contemporary cancer genomics research, including genomics, transcriptomics, microbial studies, and epigenetics. For over a decade, the ICG has maintained a record of providing updated, state-of-the-art genomic and transcriptomic services to DCI members. The ICG includes services that are performed by three Duke School of Medicine (SOM) core facilities. The Sequencing and Genomic Technologies (SGT) and Microbiome Shared Resource (MSR) perform services within the Duke Center for Genomics and Computational Biology; the Molecular Genomics Core (MGC) performs services within the Duke Molecular Physiology Institute. The ICG unifies all of the cancer genomic technologies on campus, providing one-stop access to all of the major research protocols and instrumentation platforms used in contemporary cancer genomics research. The ICG supports a wide range of projects from DCI investigators, by providing expert consultation, project management and training to facilitate access to approaches including SNP discovery, mapping chromatin modifications, single-cell sequencing, measuring mRNA levels at several scales (single genes, cancer panels, entire transcriptome), sequencing exomes, identifying DNA methylation, microbiome profiling, and mapping transcription factor binding sites. By offering the full range of technological platforms, the ICG allows investigators to choose the optimal solution for their cancer related projects and assists investigators with data quality control, versioning, statistical analysis, and dissemination for all of these services. In addition, the ICG works with DCI investigators to explore and establish new technologies which catalyzes the advancement of cancer research. In 2018, the ICG shared resource provided services to 290 investigators, 29% of whom were DCI members, accounting for 25% of total usage, from all 8 DCI Research Programs. Use of this shared resource by DCI Members contributed to 216 publications over the project period, 80 of which were in high impact journals. The shared resource operates primarily on a cost-recovery basis, with institutional support for a portion of the operating costs and instrument purchases. Support from the Cancer Center Support Grant allows ICG to provide DCI members with consultations, assistance with grant and manuscript preparation, and scheduling priority. User fees for other activities follow School of Medicine guidelines.