Flow Cytometry Core

项目总结 流式细胞仪核心 流式细胞术核心中心(FCC)一直是莫菲特癌症中心支持的共享资源机构 1990年，为莫菲特的癌症相关研究提供集中式和尖端的流式细胞仪服务。 会员可以随时方便地进入FCC，并在工作时间内提供现场协助。这个 FCC的首要目标是为会员和受训人员提供高技能和最先进的支持 在高通量和多维流式细胞仪采集和细胞分选方面提供技术援助。催化裂化 支持这一目标的活动是按照三个具体目标组织的： 目标1：在实验设计和分析方面提供专家协助。 目的2：提供最先进的流式细胞仪服务。 目标3：提供培训和教育。 FCC配备了一位久负盛名的科学总监和免疫学专家Paulo C.Rodriguez博士，以及 由经验丰富的技术专家组成的团队，他们总共拥有超过65年的研究性实践经验 在流式细胞术中。自上次竞争更新以来，FCC扩大了自己的优势，并进行了补充和升级 它的仪器。目前的FCC服务包括：1)多参数的准确检测和分析 单细胞水平；2)快速纯化目标群体的能力，评估特定细胞功能，以及 纯化细胞群体的分子特征；3)协助实验设计、数据分析和 口译；以及4)提供关于流式细胞仪技术的专家培训和教育。 值得注意的是，FCC的支持为莫菲特的多个高影响力的翻译研究项目做出了贡献。超过了 在过去的五年里，FCC的使用率增加了51%，这导致了120份同行审查的出版物，其中30份处于高水平 影响日志。此外，FCC还培训了130名研究人员和学员，并保持了一名优秀的成员 满意率每年都在90%以上。在上一财年(FY20)，FCC支持了四个成员中的61个 在五个计划中(CBE 25%，CE 3%，MM 36%，IO 36%)，其中75%的成员进行同行审查 资金问题。这占所有FCC使用量的98%。在下一个周期中，FCC将继续支持所有CCSG 计划，并根据Moffitt成员日益增长的流式细胞仪需求进一步扩展其服务。 未来FCC的优先事项是扩大其在多参数单电池中的技术和咨询服务 分析、高速细胞分选和大数据流式细胞仪工作流流程。为了满足这些需求，FCC 将获得一台新的快速细胞分选器以及一台基于光谱的细胞仪，可提供多达40+个参数 流式细胞术。最后，FCC将扩大其教育和培训举措的内容，并将制定一个流程 细胞学兴趣小组，讨论流式细胞术中的出版物、正在进行的项目和最新方法。

PROJECT SUMMARY FLOW CYTOMETRY CORE The Flow Cytometry Core (FCC) has been a Moffitt Cancer Center-supported Shared Resource Facility since 1990 and provides centralized and cutting-edge flow cytometry services for Moffitt’s cancer-related research. FCC is conveniently accessible to Members at all hours and with on-site assistance during working hours. The overarching goal of FCC is to support Members and trainees with highly skilled personnel and state-of-the-art technical assistance in high-throughput and multi-dimensional flow cytometry acquisition and cell sorting. FCC activities supporting this goal are organized intro three Specific Aims: Aim 1: To deliver expert assistance in experimental design and analysis. Aim 2: To provide state-of-the-art flow cytometry services. Aim 3: To provide training and education. FCC is staffed with a well-established Scientific Director and immunology expert, Dr. Paulo C. Rodriguez, and a team of highly experienced technologists, who collectively have more than 65 years of research-based practice in flow cytometry. Since the last competitive renewal, FCC expanded on its strengths, and added and upgraded its instrumentation. Current FCC services include: 1) Accurate detection and analysis of multiple parameters at the single-cell level; 2) Ability to rapidly purify target populations, to assess specific cellular functions, and to molecularly characterize purified cell populations; 3) Assistance in experimental design, data analysis and interpretation; and 4) Delivery of expert training and education in flow cytometry technologies. Notably, FCC support has contributed to multiple high impact translational research projects at Moffitt. Over the last five years, FCC usage increased by 51%, which resulted in 120 peer-reviewed publications, with 30 in high impact journals. Moreover, FCC trained 130 research staff and trainees, and maintained an excellent member satisfaction rate – over 90% – each year. Over the last fiscal year (FY20), FCC supported 61 Members in four of the five Programs (CBE 25%, CE 3%, MM 36%, IO 36%), with 75% of those Members holding peer review funding. This accounted for 98% of all FCC usage. In the next cycle, FCC will continue supporting all CCSG Programs and further expand its services based on the growing flow cytometry needs of Moffitt Members. Future FCC priorities are to expand its technical and consultation services in multi-parametric single-cell analysis, high-speed cell sorting, and big-data flow cytometry workflow processes. To address these needs, FCC will acquire a new fast-speed cell sorter as well as a spectral-based cytometer that provides up to 40+ parameter flow cytometry. Finally, FCC will expand its repertoire of education and training initiatives and will develop a Flow Cytometry Interest Group that discusses publications, ongoing projects, and latest approaches in flow cytometry.