Shared Resource 07: Flow Cytometry (FCSR)

项目摘要-流式细胞仪共享资源(FCSR) 流式细胞仪服务对癌症研究至关重要。由于流式细胞仪设备昂贵，而且 通常需要大量的技术专业知识，集中的资源是必不可少的。FCSR的使命是 为OSUCCC成员和高影响力的癌症研究项目提供高质量的细胞分析和 分类功能。FCSR还提供培训，并定期举办以技术为基础的研讨会或 向OSUCCC成员介绍新技术的研讨会。FCSR继续努力成为一个国家- 先进的细胞分析实验室具有以下具体目标：1)提供最先进的设备和 以收费方式为OSUCCC成员提供高质量的癌症研究支持；2)持续工作 与OSUCCC成员一起为最先进的细胞学提供丰富的技术专业知识和培训 解决癌症研究中基本问题的工具，使研究人员可以24小时访问 向OSUCCC成员介绍新的仪器、技术和 FCSR正在通过各种教育外展活动制定方法。FCSR联合- 董事是Jeffrey Chalmers，(化学和生物分子工程部)，最近被任命 联席主任，佩洛托尼亚免疫肿瘤学研究所(PIIO)副主任凯文·韦勒和 特别是开发免疫监测和发现平台，这是一项贯穿各领域的共享资源倡议 为复杂的免疫肿瘤学项目整合共享资源。此外，Gregory Behbehani博士(LR) 是Helios质量细胞仪(CyTOF)系统的教员顾问。在当前的赠款周期中，重大变化 将与PIIO的成立保持一致，这将导致 需要流式细胞仪服务。为了满足这一需求，购买了新的仪器，包括四个新的 仪器包括最先进的Cytek Aurora流式细胞仪和Helios质量细胞仪。在此期间 在此期间，FCSR贡献了231份出版物(39&>10个影响系数)，拥有296名用户，34,118小时 服务(83.7%给OSUCCC成员)，并支持80名NCI补助金(1名K12，1名K22，1名K24，6名P01，1名P50， 52个R01、1个R03、7个R21、3个R35、1个R37、1个T32和5个U01)。在下一个资金周期中，FCSR将支持 所有OSUCCC战略优先事项的需求不断增加，包括免疫肿瘤学、翻译基因组学、 癌症工程学，癌症预防和生存。用户基础将大幅增加，需要 新的工作人员和技术，例如通过购买额外的Aurora仪器和增加电池 分选、细胞分离和外体/微囊研究。此外，正在开发的新服务包括 进一步开发外切体特性和分离技术。FCSR的年度预算为 518,075美元，但CCSG的请求是108,261美元。因此，FCSR利用广泛的机构支持和 只寻求CCSG基金20.9%的支持。

PROJECT SUMMARY – FLOW CYTOMETRY SHARED RESOURCE (FCSR) Flow cytometry services are critical for cancer research. Since flow cytometry instrumentation is expensive, and typically requires significant technical expertise, a centralized resource is essential. The mission of the FCSR is to support OSUCCC members and high-impact cancer research projects with high quality cell analysis and sorting capabilities. The FCSR also provides training and regularly host technology-based seminars or workshops to introduce new technology to OSUCCC members. The FCSR continues to strive to be a state-of- the-art cell analysis laboratory that has the following Specific Aims to: 1) provide state-of-the-art equipment and support for high-quality cancer research for OSUCCC members on a fee-for-service basis; 2) continuously work with OSUCCC members to provide substantial technical expertise and training for state-of the-art cytometry instruments to address fundamental questions in cancer research so that researchers can have 24-hour access to flow cytometry instrumentation; and 3) introduce OSUCCC members to new instrumentation, technology and methodologies being developed at the FCSR through a variety of educational outreach activities. The FCSR co- Directors are Jeffrey Chalmers, (Department of Chemical and Biomolecular Engineering), and recently appointed co-Director, Kevin Weller, Associate Director of the Pelotonia Institute of Immuno-Oncology (PIIO) and specifically to develop the Immune Monitoring and Discovery Platform, a cross-cutting shared-resource initiative that integrates shared resources for complex immuno-oncology projects. Further, Dr. Gregory Behbehani (LR) is a Faculty Advisor for the Helios mass cytometer (CyTOF) system. Over the current grant cycle, major changes to the FCSR were to align with the establishment of the PIIO, which will result in a substantial increase in the need for flow cytometry services. To address this need, new instrumentation was purchased including four new instruments including a state-of-the-art Cytek Aurora flow cytometer and a Helios mass cytometer. During this time period, the FCSR contributed to 231 publications (39 >10 impact factor), had 296 users with 34,118 hours of service (83.7% to OSUCCC members), and supported 80 NCI grants (1 K12, 1 K22, 1 K24, 6 P01s, 1 P50, 52 R01s, 1 R03, 7 R21s, 3 R35s, 1 R37, 1 T32, and 5 U01s). In the next funding cycle, the FCSR will support the increasing needs of all OSUCCC strategic priorities, including immuno-oncology, translational genomics, cancer engineering, and cancer prevention and survivorship. The user base will substantially increased requiring new staff and technologies, for example by purchasing an additional Aurora instrument, and increasing cell sorting, cell isolation, and exosome/microvesicle research. In addition, new services under development include development of further exosome characterization and isolation technology. The annual budget of the FCSR is $518,075, yet the CCSG request is $108,261. As such, the FCSR leverages extensive institutional support and seeks only 20.9% support from CCSG funds.