Investigating the role of sleep in synaptic reorganization after neural injury

项目摘要 睡眠被认为是一种神经可塑性增强的状态，但支配重组的规则 在睡眠中仍然是未知的。为了更好地识别睡眠期间突触被修剪的条件， 我们已经开始研究果蝇对神经损伤的反应。触角横切后，飞得很快 在恢复到基线水平之前，增加他们的睡眠时间，最多一天。我们的初步数据还显示 在触角损伤后，突触前活动区的移除速度比质膜快，而且 损伤后的睡眠剥夺阻止了受损伤的嗅觉感受器神经元对突触前的清除。 在这个项目中，我们将研究：(1)受伤后产生的促进睡眠的信号；(2)贡献 神经胶质细胞类型对睡眠调节和损伤后突触移除的影响；(3)分子机制 促进睡眠中突触的移除。我们预计，这些实验将为我们深入了解 睡眠对神经创伤的反应，并开始调查睡眠中断的后果 轴突切断后的恢复。

Project Summary Sleep has been identified as a state of heightened neural plasticity, but the rules that govern reorganization during sleep remain unknown. To better identify the conditions under which synapses are pruned during sleep, we have begun to examine responses to neural injury in the fruit fly. After antennal transection, flies acutely increase their sleep for up to one day before returning back to baseline levels. Our preliminary data also show that pre-synaptic active zones are removed more rapidly than plasma membrane after antennal injury, and that sleep deprivation after injury prevents the clearance of pre-synapses from injured olfactory receptor neurons. In this project, we will examine: (1) signals that are generated after injuries to promote sleep; (2) contributions of glial cell types to sleep regulation and synapse removal after injury; and (3) molecular mechanisms that promote synapse removal during sleep. We anticipate that these experiments will provide insight into the role for sleep in response to neural trauma, and begin to investigate the consequences of disrupted sleep on recovery from axotomy.