Consolidated Biomedical Core Facilities Supporting a Center on Emerging and Zoonotic Infectious Disease Research

摘要：堪萨斯州立大学（KSU）处于有利地位，成为卓越的机构，以推进 发现和制定针对新发传染病和人畜共患传染病的生物安全战略。与 KSU的生物安全研究所（BSL-3），美国农业部的节肢动物传播的动物疾病研究单位， 美国农业部的国家生物和农业生物防御设施（BSL-3/BSL-4），KSU是美国唯一一所拥有完整的大学。 生物安全1级（BSL-1）至BSL-4设施的连续统一体在一个校园内配置。拟议的核心 研究设施是我们支持传染病研究基础设施的重要组成部分 研究和提供直接支持都KSU的2020年COBRE中心和配置联邦设施。核心 该设施将战略性地结合联合收割机五个关键学科：动物模型/病理学，分子和细胞生物学， 显微成像、流式细胞术和细胞分选以及下一代测序。KSU的调查人员和 在该地区依靠这些技术进行高影响力的研究和完成实验概述 目前和未来的研究项目。然而，这些关键技术的现有设施不足， 尺寸、位置、功能和灵活性。现有实验室相互隔离，分布在三个 建筑物，在某些情况下，由个别教师主持，这限制了访问和研究 生产力拟议的核心设施套件是KSU三阶段改造的最后一部分 兽医综合体第一阶段提供了一个现代的220个座位的礼堂（8,200 GSF）毗邻莫西尔大厅。 第二阶段用初级保健临床培训设施取代了一个过时的礼堂， 莫西尔大厅第三阶段，这个应用程序的重点，建议建立一个协作，大学范围内的核心 研究设施占据了被拆除的礼堂空间的二楼。目前，第二层是5000 实验室条件下壳体空间的GSF。这项建议将加强我们的研究能力和基础设施 通过建立一个集中组织的综合技术管道，并提供近距离的专家援助和培训， 提供支助，以促进在共聚焦显微镜、活细胞和体内成像方面有效利用当代技术， 激光捕获显微切割、流式细胞术、细胞分选、DNA/RNA测序和CRISPR技术。的 拟议的专用核心设施套件将在一个地点组装最先进的技术， 为学术、企业和联邦研究人员提供高效、协调的成像和分子生物学服务。 分析，从而提供从整个组织到单细胞核酸分析的全方位服务。 拥有先进仪器和技术支持的专用现代生物医学研究设施将促进 协作、协同和跨学科的科学。这对于促进强有力的研究和培训至关重要 在这里，研究人员可以回答我们这个时代最具挑战性和紧迫的生物医学问题。

SUMMARY: Kansas State University (KSU) is well-positioned to become the preeminent institution to advance the discovery and development of biosecurity strategies for emerging and zoonotic infectious diseases. With KSU’s Biosecurity Research Institute (BSL-3), USDA’s Arthropod-Borne Animal Disease Research Unit, and USDA’s National Bio and Agro-Biodefense Facility (BSL-3/BSL-4), KSU is the only U.S. university with a full continuum of biosecurity level 1 (BSL-1) through BSL-4 facilities collocated on one campus. The proposed core research facility represents a critical component of our research infrastructure to support infectious disease studies and provide direct support of both KSU’s 2020 COBRE center and collocated federal facilities. The core facility will strategically combine five key disciplines: animal model/pathology, molecular and cellular biology, microscopic imaging, flow cytometry and cell sorting, and next-generation sequencing. Investigators at KSU and in the region rely on these technologies to perform high-impact research and complete experiments outlined in current and future research projects. However, existing facilities for these key technologies are inadequate in size, location, function, and flexibility. Existing laboratories are isolated from each other, spread across three buildings, and in some cases, are hosted by individual faculty members, which limits access and research productivity. The proposed core-facility suite is the final element of a three-phase renovation of the KSU veterinary complex. Phase 1 delivered a contemporary 220-seat auditorium (8,200 GSF) adjacent to Mosier Hall. Phase 2 replaced an outdated auditorium with a primary-care clinical training facility occupying the 1st floor of Mosier Hall. Phase 3, the focus of this application, proposes to build a collaborative, university-wide core research facility occupying the 2nd floor of the deconstructed auditorium space. Currently, the 2nd floor is 5,000 GSF of laboratory-conditioned shell space. This proposal will strengthen our research capacity and infrastructure by creating a centrally organized, integrated technology pipeline, with proximate expert assistance and training support to facilitate efficient use of contemporary technology in confocal microscopy, live-cell and in vivo imaging, laser capture microdissection, flow cytometry, cell sorting, DNA/RNA sequencing and CRISPR technology. The proposed purpose-built, core-facility suite will assemble state-of-the-art technologies in a single location to deliver efficient, coordinated services for academic, corporate and federal researchers in imaging and molecular analyses, thereby providing a complete range of services from whole tissues to single-cell nucleic acid analyses. A dedicated modern biomedical research facility with advanced instrumentation and technical support will foster collaborative, synergistic and transdisciplinary science. This is critical to promoting a robust research and training environment where researchers can answer the most challenging and urgent biomedical questions of our time.