Integrators of Metastatic Potential

转移导致乳腺癌和其他实体瘤患者的主要发病率和几乎所有的死亡率。 肿瘤的我们的初步研究指出，细胞内信号分子GIV是一个中心决定因素， 乳腺癌细胞的转移潜力。GIV整合了来自多种生物化学和机械的信号 输入，将该分子作为驱动转移的关键过程的中心枢纽。在父RO1中，我们 建议通过结合基于细胞的 测定和小鼠模型。我们希望这些研究能将GIV作为一种潜在的预测生物标志物， 乳腺癌的治疗靶点。研究再入境补充将支持申请人和她的回报 一个研究生涯。申请人将参与拟议研究的所有方面，包括规划 实验、分析数据和发表手稿。她将学习新的实验方法， 多尺度，定量显微镜，以补充她在细胞和分子生物学现有的技能。的 补充将使申请人成功地过渡到一个成功的职业生涯在生物医学 research.

Metastases cause major morbidities and nearly all of deaths in patients with breast cancer and other solid tumors. Our preliminary studies point to an intracellular signaling molecule, GIV, as a central determinant of metastatic potential of breast cancer cells. GIV integrates signals from multiple biochemical and mechanical inputs, placing this molecule as a central hub for key processes driving metastasis. In the parental RO1, we propose to investigate functions of GIV in breast cancer metastasis through a combination of cell-based assays and mouse models. We expect these studies to establish GIV as a potential predictive biomarker and therapeutic target for breast cancer. The research re-entry supplement will support the applicant and her return to a research career. The applicant will participate in all aspects of the proposed research, including planning experiments, analyzing data, and publishing manuscripts. She will learn new experimental methods focused on multi-scale, quantitative microscopy to complement her existing skills in cell and molecular biology. The supplement will enable the applicant to successfully transition back into a successful career in biomedical research.